TRAM-34
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $780 | Check With Us |
| 1g | $1250 | Check With Us |
| 5g | $3375 | Check With Us |
: Cat #: V1674 CAS #: 289905-88-0 Purity ≥ 98%
Description: TRAM-34 (TRAM34; TRAM 34 Triarylmethane-34) is an inhibitor of Ca2+-activated K+ channel (IKCa1, KCa3.1) with important biological activity.
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- Physicochemical and Storage Information
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- Related Biological Data
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| Molecular Weight (MW) | 344.84 |
|---|---|
| Molecular Formula | C22H17ClN2 |
| CAS No. | 289905-88-0 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 0.4 mg/mL (1.15 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: <1 mg/mL | |
| Solubility In Vivo | 30% PEG400+0.5% Tween80+5% Propylene glycol: 30mg/mL |
| Synonyms | Triarylmethane-34 |
| Protocol | In Vitro | The TRAM-34 selectively blocks the IKCa1 current (Kd=25 nM), TRAM-34 also blocks IKCa84 current in human T1 colonic epithelial cells, with equal potency (Kd= 22 nM)。 TRAM-34 inhibits clonal and native IKCa1 channels in human T lymphocytes, Kd 20-25 nM and 200 to 1500 times more selective than other ion channels. The dose-response curve shows K for 1 μM calcium in a pipetted is 20±3 nM with a Hill coefficient of 1.2. |
|---|---|---|
| In Vivo | In the 7-day study, mice (n=5) were given a single intravenous dose of TRAM-34 (0.5 mg/kg; 29 μM) is clinically normal. Weight data for the TRAM-34 treatment group (Day 1: 17.8 g; Day 7: 27.0 g) with solvent-injected control mice (Day 1: 17.4 g; Day 7: 23.4 g) Similar. Overall, data from these limited toxicity studies suggest that TRAM-34 has no acute toxicity at 500-1,000 times the channel blocking dose at ≈<>-<>,<> times. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.8999 mL | 14.4995 mL | 28.9990 mL | 57.9979 mL |
| 5mM | 0.5800 mL | 2.8999 mL | 5.7998 mL | 11.5996 mL |
| 10mM | 0.2900 mL | 1.4499 mL | 2.8999 mL | 5.7998 mL |
| 20mM | 0.1450 mL | 0.7250 mL | 1.4499 mL | 2.8999 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
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Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.