TCID

This product is for research use only, not for human use. We do not sell to patients.

TCID
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
100mg$560Check With Us
500mg$1550Check With Us
1g$2325Check With Us

Cat #: V1325 CAS #: 30675-13-9 Purity ≥ 98%

Description: TCID (4,5,6,7-tetrachloro-1H-indene-1,3(2H)-dione) is a novel and potent DUB (deubiquitinylating enzymes) inhibitor that inhibits ubiquitin C-terminal hydrolase L3 with an IC50 of 0.6 μM.

References: Liu Y, et al. Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cellline. Chem Biol. 2003 Sep;10(9):837-46.

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Molecular Weight (MW)283.92
Molecular FormulaC9H2Cl4O2
CAS No.30675-13-9
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 23 mg/mL (81.0 mM)r
Water:<1 mg/mLr
Ethanol: <1 mg/mL
Synonyms4,5,6,7-tetrachloro-1H-indene-1,3(2H)-dione
ProtocolIn VitroTCID (4,5,6,7-Tetrachloroindan-1,3-dione; compound 11) inhibits UCH-L1 with an IC50 of 75 μM
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.5221 mL17.6106 mL35.2212 mL70.4424 mL
5mM0.7044 mL3.5221 mL7.0442 mL14.0885 mL
10mM0.3522 mL1.7611 mL3.5221 mL7.0442 mL
20mM0.1761 mL0.8805 mL1.7611 mL3.5221 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.