SPDB crosslinker

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$1000	To Be Confirmed
500g	$1500	To Be Confirmed
1g	$2250	To Be Confirmed

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Cat #: V5622 CAS #: 115088-06-7 Purity ≥ 98%

Description: SPDB is useful heterobifunctional cross-linker, which is widely used to conjugate a small molecule drug to a targeted carrier (such as antibody, enzyme, protein and polymer). SPDB can be used to make ADC (antobody drug conjugate. Conjugated to antibodies utilizing anti-EGFR-SPDB-DM4 has been widely used.

References: [1]. Widdison WC et al. Development of Anilino-Maytansinoid ADCs that Efficiently Release Cytotoxic Metabolitesin Cancer Cells andInduce High Levels of Bystander Killing. Bioconjug Chem, 2015 Nov 18, 26(11):2261-78.

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Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	326.39
Molecular Formula	C13H14N2O4S2
CAS No.	115088-06-7
SMILES Code	O=C(ON1C(CCC1=O)=O)CCCSSC2=NC=CC=C2
Synonyms	SPDB disulfide heterobifunctional cross-linker; SPDB;

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.0638 mL	15.3191 mL	30.6382 mL	61.2764 mL
5mM	0.6128 mL	3.0638 mL	6.1276 mL	12.2553 mL
10mM	0.3064 mL	1.5319 mL	3.0638 mL	6.1276 mL
20mM	0.1532 mL	0.7660 mL	1.5319 mL	3.0638 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.