SPAUTIN-1
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $280 | Check With Us |
| 500mg | $450 | Check With Us |
| 1g | $675 | Check With Us |
: Cat #: V2534 CAS #: 1262888-28-7 Purity ≥ 98%
Description: Spautin-1 is a specific autophagy inhibitor which inhibits the deubiquitinating activity of USP10 and USP13 with IC50 of ∼0.6-0.7 μM.
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| Molecular Weight (MW) | 271.26 |
|---|---|
| Molecular Formula | C15H11F2N3 |
| CAS No. | 1262888-28-7 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 54 mg/mL (199.1mM)r |
| Water: < 1 mg/mLr | |
| Ethanol: 7 mg/mL (25.8 mM) | |
| SMILES Code | FC1=CC=C(CNC2=C3C=C(F)C=CC3=NC=N2)C=C1 |
| Synonyms | Spautin-1; Spautin 1; Spautin1 |
| Protocol | In Vitro | Spautin-1 enhances imatinib mesylate (IM)-induced CmL cell apoptosis by reducing the expression of the anti-apoptotic proteins Mcl-1 and Bcl-2. The pro-apoptotic activity of spautin-1 is associated with activation of GSK3β, an important downstream effector of PI3K/AKT. Spautin-1 enhances IM-induced cytotoxicity in CmL cell line K562, decreasing the IC50 from 1 to 0.5 μM. |
|---|---|---|
| In Vivo | Spautin-1 ameliorates the pathogenesis of acute pancreatitis induced by cerulein or L-arginine. Spautin-1 pretreatment significantly diminishes the elevation of serum amylase and lipase levels, which are indicative of trypsin activity. Increasing levels of serum TNFα caused by cerulein are inhibited in the presence of spautin-1. Spautin-1 treatment can ameliorate the inflammation damage induced by cerulein, such as edema, degeneration, coagulative necrosis and infiltration of inflammatory cells. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.6865 mL | 18.4325 mL | 36.8650 mL | 73.7300 mL |
| 5mM | 0.7373 mL | 3.6865 mL | 7.3730 mL | 14.7460 mL |
| 10mM | 0.3687 mL | 1.8433 mL | 3.6865 mL | 7.3730 mL |
| 20mM | 0.1843 mL | 0.9216 mL | 1.8433 mL | 3.6865 mL |
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Method for preparing in vivo formulation:
Take
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PEG300, mix and clarify, next add
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Tween 80,mix and clarify, next add
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ddH2O,mix and clarify.
Note:
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