RO2959 HCl
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $1550 | Check With Us |
| 200mg | $2325 | Check With Us |
| 500mg | $3925 | Check With Us |
: Cat #: V13927 CAS #: 1219927-22-6 Purity ≥ 99%
Description: RO2959 is a novel, potent and highly selective CRAC current inhibitor (IC50 of 402 nM), also a potent blocker of store operated calcium entry (SOCE) mediated by Orai1/Stim1 channels with an IC50 of 25 nM. .
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| Molecular Weight (MW) | 463.9318 |
|---|---|
| Molecular Formula | C21H20ClF2N5OS |
| CAS No. | 1219927-22-6 |
| SMILES Code | O=C(NC1=NC=C(C2=C(C)CCN(C3=NC=C(C)S3)C2)N=C1)C4=C(F)C=CC=C4F.[H]Cl |
| Synonyms | RO2959; RO 2959; RO2959 Hydrochloride; RO2959 HCl; RO-2959; |
| Protocol | In Vitro | RO2959 is a potent SOCE inhibitor that blocks an IP3-dependent current in CRAC-expressing RBL-2H3 cells and CHO cells stably expressing human Orai1 and Stim1, as well as SOCE in human primary CD4+ T cells triggered by either T cell receptor (TCR) stimulation or thapsigargin treatment. RO2959 completely inhibits cytokine production as well as T cell proliferation mediated by TCR stimulation or MLR (mixed lymphocyte reaction). RO2959 inhibits Orai1 and Orai3 with IC50 values of 25 nM and 530 nM, respectively. RO2959 blocks store operated calcium entry (SOCE) in activated CD4+T lymphocytes with an IC50 value of 265 nM. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.1555 mL | 10.7774 mL | 21.5549 mL | 43.1098 mL |
| 5mM | 0.4311 mL | 2.1555 mL | 4.3110 mL | 8.6220 mL |
| 10mM | 0.2155 mL | 1.0777 mL | 2.1555 mL | 4.3110 mL |
| 20mM | 0.1078 mL | 0.5389 mL | 1.0777 mL | 2.1555 mL |
The molarity calculator equation
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.