RITA (NSC 652287)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $925 | Check With Us |
| 500mg | $1280 | Check With Us |
| 1g | $1920 | Check With Us |
: Cat #: V0015 CAS #: 213261-59-7 Purity ≥ 98%
Description: RITA (also known as NSC-652287) is a novel and potent inhibitor of p53-HDM-2 protein-protein interaction with potential anticancer activity.
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- Physicochemical and Storage Information
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- Related Biological Data
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| Molecular Weight (MW) | 292.4 |
|---|---|
| Molecular Formula | C14H12O3S2 |
| CAS No. | 213261-59-7 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 58 mg/mL (198.37 mM)r |
| Water:<1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: 8 mg/mL (27.36 mM) | |
| Solubility In Vivo | 30% Propylene glycol, 5% Tween 80, 65% D5W: 30 mg/mL |
| SMILES Code | OCC1=CC=C(C2=CC=C(C3=CC=C(CO)S3)O2)S1 |
| Synonyms | NSC-652287; NSC652287; NSC 652287 |
| Protocol | In Vitro | RITA inhibits p53-HDM-2 interaction, binding to p53dN, with a Kd of 1.5 nM. RITA (10 μM) blocks complex formation between p53 and HDM-2 in HCT116 cells and HDFs and in NHF-ERMyc cells irrespective of c-Myc expression. RITA (0.5 μM) reduces the viability of tumor cells in a wild-type p53-dependent manner. Moreover, RITA (0.1 μM) induces p53-dependent apoptosis. RITA induces p53 but does not via DNA damage-signaling pathway. |
|---|---|---|
| In Vivo | RITA (0.1, 1 or 10 mg/kg, i.p.) shows potent antitumor activity in SCID mice bearing HCT116 and HCT116 TP53−/− xenografts. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.4200 mL | 17.0999 mL | 34.1997 mL | 68.3995 mL |
| 5mM | 0.6840 mL | 3.4200 mL | 6.8399 mL | 13.6799 mL |
| 10mM | 0.3420 mL | 1.7100 mL | 3.4200 mL | 6.8399 mL |
| 20mM | 0.1710 mL | 0.8550 mL | 1.7100 mL | 3.4200 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
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Concentration
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Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
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Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.