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PSI-6130

This product is for research use only, not for human use. We do not sell to patients.

PSI-6130
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
250mg$1950Check With Us
500mg$2950Check With Us
1g$4425Check With Us
email: [email protected]        [email protected]

Cat #: V3664 CAS #: 817204-33-4 Purity ≥ 98%

Description: PSI-6130 (R-1656) is a novel, potent and selective inhibitor of HCV NS5B polymerase which inhibits HCV replication with a mean IC50 of 0.6 μM. PSI-6130 is a cytidine analogue with potent and selective anti-hepatitis C virus (HCV) activity in the subgenomic HCV replicon assay, 90% effective concentration (EC90)=4.6 +/- 2.0 microM. The spectrum of activity and cytotoxicity profile of PSI-6130 was evaluated against a diverse panel of viruses and cell types, and against two additional HCV-1b replicons.

References: Ali S, et al. Selected replicon variants with low-level in vitro resistance to the hepatitis C virus NS5B polymerase inhibitor PSI-6130 lack cross-resistance with R1479. Antimicrob Agents Chemother. 2008 Dec;52(12):4356-69.

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Molecular Weight (MW)259.2374
Molecular FormulaC10H14FN3O4
CAS No.817204-33-4
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
SMILES CodeOC[C@@H]1[C@H]([C@](C)(F)[C@H](N2C(N=C(C=C2)N)=O)O1)O
SynonymsR 1656; R-1656; R1656; PSI-6130; PSI-6130; PSI-6130;
ProtocolIn VitroPSI-6130 exhibits potent and specific inhibitory activity against HCV RNA replication mediated by the NS5B polymerase. Both PSI-6130 inhibit HCV GT-1b (Con1 strain) and GT-1a (H77 strain) subgenomic RNA replication, with mean EC50 values of 0.51 and 0.30 μM, respectively. PSI-6130 inhibits 40% human serum with EC50 of 0.51 μM. PSI-6130 inhibits HCV replication with a mean IC50 of 0.6 μM, PSI-6130-TP inhibits HCV replicase with a mean IC50 of 0.34 μM. PSI-6130-TP inhibits recombinant HCV Con1 NS5B on a heteropolymeric RNA template derived from the 3′-end of the negative strand of the HCV genome with an IC50 of 0.13 μM and Ki of 0.023 μM.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.8575 mL19.2873 mL38.5747 mL77.1494 mL
5mM0.7715 mL3.8575 mL7.7149 mL15.4299 mL
10mM0.3857 mL1.9287 mL3.8575 mL7.7149 mL
20mM0.1929 mL0.9644 mL1.9287 mL3.8575 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.