PNU-74654
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $750 | Check With Us |
| 500mg | $1250 | Check With Us |
| 1g | $1875 | Check With Us |
: Cat #: V27702 CAS #: 113906-27-7 Purity ≥ 99%
Description: PNU-74654 (PNU74654) is a novel inhibitor of Wnt/β-catenin pathway (IC50 = 129.8 μM), acting by inhibiting the interaction between ß-catenin and T cell factor 4 (Tcf4), and disrupting the Wnt signaling pathway.
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- Physicochemical and Storage Information
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- Related Biological Data
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| Molecular Weight (MW) | 320.34 |
|---|---|
| Molecular Formula | C19H16N2O3 |
| CAS No. | 113906-27-7 |
| Protocol | In Vitro | PNU-74654 binds to β-catenin with a KD of 450 nM. The Tcf3/Tcf4-binding surface on β-catenin contains a well-defined hot spot around residues K435 and R469. The binding mode of PNU-74654 involves the two narrow pockets on either side of this hot spot |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.1217 mL | 15.6084 mL | 31.2168 mL | 62.4337 mL |
| 5mM | 0.6243 mL | 3.1217 mL | 6.2434 mL | 12.4867 mL |
| 10mM | 0.3122 mL | 1.5608 mL | 3.1217 mL | 6.2434 mL |
| 20mM | 0.1561 mL | 0.7804 mL | 1.5608 mL | 3.1217 mL |
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.