Pifithrin-μ (NSC-303580)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $775 | Check With Us |
| 1g | $1275 | Check With Us |
| 5g | $3225 | Check With Us |
: Cat #: V0020 CAS #: 64984-31-2 Purity ≥ 98%
Description: Pifithrin-μ (NSC303580) is a novel and potent inhibitor of p53 binding and p53-mediated apoptosis with a Kd of 0.82 mM in vitro.
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- Physicochemical and Storage Information
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- Related Biological Data
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| Molecular Weight (MW) | 181.21 |
|---|---|
| Molecular Formula | C8H7NO2S |
| CAS No. | 64984-31-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 36 mg/mL (198.7 mM)r |
| Water: <1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: N/A | |
| Solubility In Vivo | 1% DMSO+30% polyethylene glycol+1% Tween 80:10 mg/mL |
| Synonyms | Pifithrin-µ; Pifithrin µ; Pifithrinµ; 2-Phenylethynesulfonamide; PFT-µ; PFT µ; PFTµ; NSC303580; NSC-303580; NSC 303580 |
| Protocol | In Vitro | Pifithrin-μ (10 μM) is a p53 inhibitor, which inhibits p53 binding to mitochondria by reducing its affinity to antiapoptotic proteins Bcl-xL and Bcl-2 but has no effect on p53-dependent transactivation, activity of caspases 2, 8, 9 and 10 in a cell-free system, or NF-κB-dependent transcription. |
|---|---|---|
| In Vivo | Pifithrin-μ (40 mg/kg, i.p.) shows no protective effect against doses of radiation that cause gastrointestinal syndrome in mice. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 5.5185 mL | 27.5923 mL | 55.1846 mL | 110.3692 mL |
| 5mM | 1.1037 mL | 5.5185 mL | 11.0369 mL | 22.0738 mL |
| 10mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | 11.0369 mL |
| 20mM | 0.2759 mL | 1.3796 mL | 2.7592 mL | 5.5185 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.