PFK15
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $675 | Check With Us |
| 500mg | $1012 | Check With Us |
| 1g | $1510 | Check With Us |
: Cat #: V1937 CAS #: 4382-63-2 Purity ≥ 98%
Description: PFK-015 (also known as PFK15) is a novel, potent and selective 6-phosphofructo-2-kinase (PFKFB3) inhibitor with IC50 of 207 nM.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 260.29 |
|---|---|
| Molecular Formula | C17H12N2O |
| CAS No. | 4382-63-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 19 mg/mL (73 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: 2 mg/mL (7.67mM) | |
| Solubility In Vivo | 5% DMSO+45% PEG 300+1% Tween 80+ddH2O: 5mg/mL |
| Synonyms | PFK-015; PFK 015; PFK015. |
| Protocol | In Vitro | PFK-015 inhibits tumor growth in a dose-dependent manner in esophageal cancer cell line in vitro. PFK-015 (0-5 μM) increases HIF-1α mediated PD-L1 transcriptional activity. PFK-015 induces the expression of tumor PD-L1 via the phos-PFKFB3/HIF-1a axis. |
|---|---|---|
| In Vivo | PFK-015 impeds ESCC tumor growth in immunodeficient in vivo models. PFK-015 (0-12.5 μM, 48 h) induces tumor PD-L1 expression[1]. PFK-015 (0-12.5 μM, 48 h) can cause a downregulation of immune activity against tumor cells mediated by CD8 + T cells. PFK-015 enhances the efficacy of ESCC by enhancing CD8 + T-cell activity combining PD-1 mAb in immunocompetent mouse models such as C57BL/6 and hu-PBMC-NOG. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.8419 mL | 19.2093 mL | 38.4187 mL | 76.8374 mL |
| 5mM | 0.7684 mL | 3.8419 mL | 7.6837 mL | 15.3675 mL |
| 10mM | 0.3842 mL | 1.9209 mL | 3.8419 mL | 7.6837 mL |
| 20mM | 0.1921 mL | 0.9605 mL | 1.9209 mL | 3.8419 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.