PF-3450074 | CAS 1352879-65-2

PF-3450074

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$879	Check With Us
500mg	$1270	Check With Us
1g	$1900	Check With Us

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Cat #: V3323 CAS #: 1352879-65-2 Purity ≥ 98%

Description: PF-3450074 (also known as PF74) is a HIV-1 capsid-targeting small molecule antiviral compound which directly binds to the capsid (CA) protein at a site also utilized by host cell proteins CPSF6 and NUP153. The RNA genome of human immunodeficiency virus type 1 (HIV-1) is enclosed in a cone-shaped capsid shell that disassembles following cell entry via a process known as uncoating. During HIV-1 infection, the capsid is important for reverse transcription and entry of the virus into the target cell nucleus. The small molecule PF74 inhibits HIV-1 infection at early stages by binding to the capsid and perturbing uncoating.

References: Xu JP, et al. Exploring Modifications of an HIV-1 Capsid Inhibitor: Design, Synthesis, and Mechanism of Action.J Drug Des Res. 2018;5(2). pii: 1070. Epub 2018 Aug 13.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	425.53
Molecular Formula	C27H27N3O2
CAS No.	1352879-65-2
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 10 mMr
	Water: N/Ar
	Ethanol: N/A
SMILES Code	O=C(N[C@@H](CC1=CC=CC=C1)C(N(C)C2=CC=CC=C2)=O)CC3=C(C)NC4=C3C=CC=C4
Synonyms	PF-3450074; PF 3450074; PF3450074; PF74; PF-74; PF 74

Protocol	In Vitro	PF-3450074 (PF-74) exhibits anti-viral activities against HIV wild type NL4-3 and HIV T107N mutant with EC50 values of 0.72 μM and 4.5μM, respectively.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Related Biological Data

Topographic AFM images of the morphological changes that HIV-1 cores treated with 10 μM PF74 undergo during reverse transcription. J Virol. 2018 Sep 26;92(20). pii: e00845-18.

High-resolution spatial mechanical mapping of an HIV-1 core surface treated with 10 μM PF74 during reverse transcription. J Virol. 2018 Sep 26;92(20). pii: e00845-18.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.3500 mL	11.7501 mL	23.5001 mL	47.0002 mL
5mM	0.4700 mL	2.3500 mL	4.7000 mL	9.4000 mL
10mM	0.2350 mL	1.1750 mL	2.3500 mL	4.7000 mL
20mM	0.1175 mL	0.5875 mL	1.1750 mL	2.3500 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.