NSC23005 sodium

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NSC23005 sodium
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Size Price Stock
5mg$653-6 Days
10mg$953-6 Days
25mg$1353-6 Days
50mg$2353-6 Days
100mg$4193-6 Days
250mg$6803-6 Days
500mg$11503-6 Days

Cat #: V3274 CAS #: 1796596-46-7 (NSC23005 sodium); 6314-70-1 (NSC23005 free acid) Purity ≥ 98%

Description: NSC23005 sodium is a novel and potent p18 inhibitor with ED50 of 5.21 nM in promoting Hematopoietic stem cells (HSCs) expansion in both murine and human models. Hematopoietic stem cells have emerged as promising therapeutic cell sources for high-risk hematological malignancies and immune disorders. However, their clinical use is limited by the inability to expand these cells ex vivo. Therefore, there is an urgent need to identify specific targets and effective probes that can expand HSCs. NSC23005 sodium represents novel chemical agents for murine and human HSCs ex vivo expansion and also can be used as valuable chemical probes for further HSC biology research towards promising utility for therapeutic purposes.

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Molecular Weight (MW)305.32
Molecular FormulaC13H16NNaO4S
CAS No.1796596-46-7 (NSC23005 sodium); 6314-70-1 (NSC23005 free acid)
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 6.5 mg/mL
Water:
Ethanol:
SMILES CodeO=C([O-])C1=CC=C(S(=O)(NC2CCCCC2)=O)C=C1.[Na+]
SynonymsNSC23005; NSC-23005; NSC 23005; p18SMI-40; NSC23005 sodium
ProtocolIn VitroIn vitro activity: NSC23005 sodium is a novel and potent p18 inhibitor with ED50 of 5.21 nM in promoting Hematopoietic stem cells (HSCs) expansion in both murine and human models. Hematopoietic stem cells have emerged as promising therapeutic cell sources for high-risk hematological malignancies and immune disorders. However, their clinical use is limited by the inability to expand these cells ex vivo. Therefore, there is an urgent need to identify specific targets and effective probes that can expand HSCs. NSC23005 sodium represents novel chemical agents for murine and human HSCs ex vivo expansion and also can be used as valuable chemical probes for further HSC biology research towards promising utility for therapeutic purposes. NSC23005 sodium is a novel class of INK4C (p18INK4C or p18) small molecule inhibitor (p18SMIs), which is initially found by in silico 3D screening. NSC23005 sodium shows the most potent bioactivity in hematopoietic stem cells (HSCs) expansion (ED50=5.21 nM). Notably, NSC23005 sodium does not show significant cytotoxicity toward 32D cells or HSCs, nor does it augment leukemia cell proliferation. NSC23005 sodium (ED50=5.21 nM), shows no activity in promoting the proliferation of leukemia cells. Kinase Assay: rior to culture, cKit-enriched BM cells (1 × 106/mL) were labeled with 5- (and 6-) carboxy-fluorescein diacetatesuccinimidylester (CFSE) dye (3 μM, Molecular Probes) in PBS supplemented with 0.1% bovine serum albumin. Labeling was performed in the dark at 37 °C for 10 min. Labeling was stopped by the addition of 5 volumes of ice cold PBS. 4 days after culture with cytokine plus compound, labeled cells were harvested and stained with the antibody cocktail for lineage markers, Sca-1, CD48, and CD150. During cell division, CFSE is distributed equally between daughter cells so that the generation of cells could be reflected by the content of CFSE and measured by the intensity of fluorescence. The CyAnsystem (DakoCytomation) was used for data acquisition. The data was analyzed using cells/Proliferation module of FlowJo software (Treestar, Inc.), which would fit a curve of the input data, automatically generate peaks standing for subpopulations with different fluorescence intensity and calculate the percentage of each peak and divided statistics results. Cell Assay: c-Kit enriched bone marrow (BM) cells are cultured for 5 days with cytokine combination plus NSC23005 sodium or DMSO. As positive controls, primary uncultured bone marrow cells are treated by ultraviolet radiation (UV) for 10 minutes prior to the staining process for apoptosis analysis. Apoptosis and cell death are measured by AnnexinV and DAPI staining in the Annexin V-FITC Apoptosis Detection Kit. Apoptosis is measured on an FACS analyzer. The data is analyzed using FlowJo software.
In VivoMice (6–8 weeks old) were purchased from Jackson laboratory (Bar Harbor, ME) and housed in specific pathogen-free rooms within the animal-care facilities of the University of Pittsburgh Cancer Institute. All procedures of the mouse work were approved by the Institutional Animal Care and Use Committee at the University of Pittsburgh. HSCs expansion medium consists of BIT9500 (Stem Cell Technologies) supplemented with 50 ng/mL recombinant mouse (rm) SCF, 20 ng/mL FMS-like tyrosine kinase 3 ligand (Flt3L) and 10 ng/mL thrombopoietin (Tpo) (all from PeproTech, Inc.). p18SMI compounds were added where indicated. Bone marrow cells were harvested from C57BL/6 mice and made into a single-cell suspension. After immunomagnetic c-Kit enrichment (cKit-conjugated microbeads [MiltenyiBiotec, Bergisch-Gladbach]), cells were washed and resuspended in HSCs expansion medium. After culture, total nucleated cell counts were obtained, and a fraction of mononuclear cells (MNCs) or whole bone marrow cells were stained for flow cytometry analysis. Frequency of each cell population was determined by independently analyzing more than 5 × 105 cells per sample in triplicate.
Animal modelMice (6–8 weeks old)
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.2753 mL16.3763 mL32.7525 mL65.5050 mL
5mM0.6551 mL3.2753 mL6.5505 mL13.1010 mL
10mM0.3275 mL1.6376 mL3.2753 mL6.5505 mL
20mM0.1638 mL0.8188 mL1.6376 mL3.2753 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
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Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
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=
Concentration(final)
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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.