NSC23005
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 5mg | $70 | 3-6 Days |
| 10mg | $100 | 3-6 Days |
| 25mg | $150 | 3-6 Days |
| 50mg | $250 | 3-6 Days |
| 100mg | $439 | 3-6 Days |
| 250mg | $730 | 3-6 Days |
| 500mg | $1150 | 3-6 Days |
: Cat #: V3275 CAS #: 6314-70-1 (NSC23005 free acid); 1796596-46-7 (NSC23005 sodium) Purity ≥ 98%
Description: NSC23005 is a novel and potent p18 inhibitor with ED50 of 5.21 nM in promoting Hematopoietic stem cells (HSCs) expansion in both murine and human models. Hematopoietic stem cells have emerged as promising therapeutic cell sources for high-risk hematological malignancies and immune disorders. However, their clinical use is limited by the inability to expand these cells ex vivo. Therefore, there is an urgent need to identify specific targets and effective probes that can expand HSCs. NSC23005 sodium represents novel chemical agents for murine and human HSCs ex vivo expansion and also can be used as valuable chemical probes for further HSC biology research towards promising utility for therapeutic purposes.
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| Molecular Weight (MW) | 283.34 |
|---|---|
| Molecular Formula | C13H17NO4S |
| CAS No. | 6314-70-1 (NSC23005 free acid); 1796596-46-7 (NSC23005 sodium) |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 6.5 mg/mL |
| Water: | |
| Ethanol: | |
| SMILES Code | O=C([O-])C1=CC=C(S(=O)(NC2CCCCC2)=O)C=C1 |
| Synonyms | NSC23005; NSC-23005; NSC 23005; p18SMI-40; NSC23005 |
| Protocol | In Vitro | In vitro activity: NSC23005 sodium is a novel and potent p18 inhibitor with ED50 of 5.21 nM in promoting Hematopoietic stem cells (HSCs) expansion in both murine and human models. Hematopoietic stem cells have emerged as promising therapeutic cell sources for high-risk hematological malignancies and immune disorders. However, their clinical use is limited by the inability to expand these cells ex vivo. Therefore, there is an urgent need to identify specific targets and effective probes that can expand HSCs. NSC23005 sodium represents novel chemical agents for murine and human HSCs ex vivo expansion and also can be used as valuable chemical probes for further HSC biology research towards promising utility for therapeutic purposes. NSC23005 sodium is a novel class of INK4C (p18INK4C or p18) small molecule inhibitor (p18SMIs), which is initially found by in silico 3D screening. NSC23005 sodium shows the most potent bioactivity in hematopoietic stem cells (HSCs) expansion (ED50=5.21 nM). Notably, NSC23005 sodium does not show significant cytotoxicity toward 32D cells or HSCs, nor does it augment leukemia cell proliferation. NSC23005 sodium (ED50=5.21 nM), shows no activity in promoting the proliferation of leukemia cells. Kinase Assay: rior to culture, cKit-enriched BM cells (1 × 106/mL) were labeled with 5- (and 6-) carboxy-fluorescein diacetatesuccinimidylester (CFSE) dye (3 μM, Molecular Probes) in PBS supplemented with 0.1% bovine serum albumin. Labeling was performed in the dark at 37 °C for 10 min. Labeling was stopped by the addition of 5 volumes of ice cold PBS. 4 days after culture with cytokine plus compound, labeled cells were harvested and stained with the antibody cocktail for lineage markers, Sca-1, CD48, and CD150. During cell division, CFSE is distributed equally between daughter cells so that the generation of cells could be reflected by the content of CFSE and measured by the intensity of fluorescence. The CyAnsystem (DakoCytomation) was used for data acquisition. The data was analyzed using cells/Proliferation module of FlowJo software (Treestar, Inc.), which would fit a curve of the input data, automatically generate peaks standing for subpopulations with different fluorescence intensity and calculate the percentage of each peak and divided statistics results. Cell Assay: c-Kit enriched bone marrow (BM) cells are cultured for 5 days with cytokine combination plus NSC23005 sodium or DMSO. As positive controls, primary uncultured bone marrow cells are treated by ultraviolet radiation (UV) for 10 minutes prior to the staining process for apoptosis analysis. Apoptosis and cell death are measured by AnnexinV and DAPI staining in the Annexin V-FITC Apoptosis Detection Kit. Apoptosis is measured on an FACS analyzer. The data is analyzed using FlowJo software. |
|---|---|---|
| In Vivo | Mice (6–8 weeks old) were purchased from Jackson laboratory (Bar Harbor, ME) and housed in specific pathogen-free rooms within the animal-care facilities of the University of Pittsburgh Cancer Institute. All procedures of the mouse work were approved by the Institutional Animal Care and Use Committee at the University of Pittsburgh. HSCs expansion medium consists of BIT9500 (Stem Cell Technologies) supplemented with 50 ng/mL recombinant mouse (rm) SCF, 20 ng/mL FMS-like tyrosine kinase 3 ligand (Flt3L) and 10 ng/mL thrombopoietin (Tpo) (all from PeproTech, Inc.). p18SMI compounds were added where indicated. Bone marrow cells were harvested from C57BL/6 mice and made into a single-cell suspension. After immunomagnetic c-Kit enrichment (cKit-conjugated microbeads [MiltenyiBiotec, Bergisch-Gladbach]), cells were washed and resuspended in HSCs expansion medium. After culture, total nucleated cell counts were obtained, and a fraction of mononuclear cells (MNCs) or whole bone marrow cells were stained for flow cytometry analysis. Frequency of each cell population was determined by independently analyzing more than 5 × 105 cells per sample in triplicate. | |
| Animal model | Mice (6–8 weeks old) |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.5293 mL | 17.6466 mL | 35.2933 mL | 70.5866 mL |
| 5mM | 0.7059 mL | 3.5293 mL | 7.0587 mL | 14.1173 mL |
| 10mM | 0.3529 mL | 1.7647 mL | 3.5293 mL | 7.0587 mL |
| 20mM | 0.1765 mL | 0.8823 mL | 1.7647 mL | 3.5293 mL |
The molarity calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.