NS-398 | CAS 123653-11-2

NS-398

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$489	Check With Us
500mg	$739	Check With Us
1g	$1105	Check With Us

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Cat #: V2703 CAS #: 123653-11-2 Purity ≥ 98%

Description: NS-398 [also called NS 398, N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide] is a novel, highly potent and selective inhibitor of cyclooxygenase-2 (COX-2) that is used as a non-steroidal an-inflammatory agent with analgesic and antipyretic effects.

References: Futaki N, et al. NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro. Prostaglandins. 1994 Jan;47(1):55-9.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	314.36
Molecular Formula	C13H18N2O5S
CAS No.	123653-11-2
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 62 mg/mL (197.2 mM)r
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
SMILES Code	CS(=O)(NC1=CC=C([N+]([O-])=O)C=C1OC2CCCCC2)=O
Synonyms	NS-398; NS 398; NS398; N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

Protocol	In Vitro	NS-398 is a non-steroidal an-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity, with an IC50 of 3.8 μM, and has no effect on COX-1 at 100 μM.
Protocol	In Vivo	NS-398 (0.5-10 mg/kg, p.o.) dose-dependently inhibits paw edema in rats, with an ED30 of 1.14 mg/kg, shows therapeutic effects on adjuvant arthritis (ED30, 4.69 mg/kg), exhibits dose-dependent analgesic activity (ED50, 1.65 mg/kg), and has antipyretic effect (ED50, 1.84 mg/kg) in rats. In mice, NS-398 suppresses writhing reactions induced by acetic acid with an ED50 of 8.2 mg/kg.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.1811 mL	15.9053 mL	31.8107 mL	63.6213 mL
5mM	0.6362 mL	3.1811 mL	6.3621 mL	12.7243 mL
10mM	0.3181 mL	1.5905 mL	3.1811 mL	6.3621 mL
20mM	0.1591 mL	0.7953 mL	1.5905 mL	3.1811 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.