Necrostatin-1 (Nec-1)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $450 | In Stock |
| 1g | $650 | In Stock |
| 5g | $1645 | In Stock |
: Cat #: V0037 CAS #: 4311-88-0 Purity ≥ 98%
Description: Necrostatin-1 (Nec-1) is a novel, potent and specific RIP1 (receptor-interacting protein 1) kinase inhibitor that inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells.
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| Molecular Weight (MW) | 259.33 |
|---|---|
| Molecular Formula | C13H13N3OS |
| CAS No. | 4311-88-0 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 51 mg/mL warming (196.66 mM)r |
| Water:<1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: <1 mg/mL (slightly soluble or insoluble) | |
| SMILES Code | O=C(C(CC1=CNC2=C1C=CC=C2)N3)N(C)C3=S |
| Synonyms | Necrostatin-1; Necrostatin1; Necrostatin 1; Nec-1; Nec 1; Nec1 |
| Protocol | In Vitro | Necrostatin-1 (Nec-1) efficiently inhibits the TNFα-induced necrotic death of L929 cells, which does not require exogenous caspase inhibitors. |
|---|---|---|
| In Vivo | Necrostatin-1 (Nec-1) induces tubular bilation and affects the kinetics of the dilation of peritubular capillaries after RCM application. Upon a single intraperitoneal application of a single dose of Necrostatin-1 (1.65 mg/kg body weight, i.p.) 15 minutes before RCM, the return to baseline levels is prevented within the observation period. | |
| Animal model | Mice: 8-10 week old male C57BL/6 mice (average weight approx.23 g) are used. Mice receive intravenous application of 200 μL PBS or radiocontrast media (RCM) via the tail vein. A single dose of Z-VAD-fmk (10 mg/kg body weight) or Necrostatin-1 (1.65 mg/kg body weight) is applied intraperitoneally 15 min. before RCM-injection. Mice are harvested another 24 hours after RCM-application (48 hours after reperfusion). Blood samples are obtained from retroorbital bleeding and serum levels of urea and creatinine are determined. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.8561 mL | 19.2805 mL | 38.5609 mL | 77.1218 mL |
| 5mM | 0.7712 mL | 3.8561 mL | 7.7122 mL | 15.4244 mL |
| 10mM | 0.3856 mL | 1.9280 mL | 3.8561 mL | 7.7122 mL |
| 20mM | 0.1928 mL | 0.9640 mL | 1.9280 mL | 3.8561 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.