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NCB-0846

This product is for research use only, not for human use. We do not sell to patients.

NCB-0846
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
250mg$1150To Be Confirmed
500mg$1650To Be Confirmed
1g$2475To Be Confirmed
email: [email protected]        [email protected]

Cat #: V3200 CAS #: 1792999-26-8 Purity ≥ 98%

Description: NCB-0846 (NCB0846) is a novel, first orally bioavailable small molecule inhibitor of Wnt that inhibits TNIK (TRAF2 and NCK-Interacting Kinase) with IC50 value of 21 nM.

References: Masuda M, et al. TNIK inhibition abrogates colorectal cancer stemness. Nat Commun. 2016 Aug 26;7:12586.

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Molecular Weight (MW)375.42
Molecular FormulaC21H21N5O2
CAS No.1792999-26-8
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 75 mg/mLr
Water: < 1 mg/mLr
Ethanol: 2 mg/mL
SMILES CodeO[C@H]1CC[C@@H](OC2=CC=CC3=CN=C(NC4=CC=C5N=CNC5=C4)N=C23)CC1
SynonymsNCB 0846; NCB0846; NCB-0846
ProtocolIn VitroNCB-0846 has anti-Wnt activity. NCB-0846 binds to TNIK in an inactive conformation, and this binding mode seems to be essential for Wnt inhibition. NCB-0846 shows inhibitory activity against TNIK with an IC50 of 21 nM. NCB-0846 also inhibits FLT3, JAK3, PDGFRα, TRKA, CDK2/CycA2, and HGK. NCB-0846 induces faster migration of TCF4 phosphorylated by TNIK within a concentration range of 0.1-0.3 μM and completely inhibits the phosphorylation of TCF4 at a concentration of 3 μM. NCB-0846 inhibits HCT116 cell growth and shows much higher (-20-fold) inhibitory activity against colony formation by the same cells in soft agar
In VivoNCB-0846 suppresses the growth of tumors established by inoculating HCT116 cells into immunodeficient mice. The expression of Wnt-target genes (AXIN2, MYC and CCND1) in xenografts is reduced following the administration of NCB-0846. NCB-0846 induces an increase in the sub-G1 cell population. Cleavage of poly (ADP-ribose) polymerase 1 indicates the induction of apoptosis
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.6637 mL13.3184 mL26.6368 mL53.2737 mL
5mM0.5327 mL2.6637 mL5.3274 mL10.6547 mL
10mM0.2664 mL1.3318 mL2.6637 mL5.3274 mL
20mM0.1332 mL0.6659 mL1.3318 mL2.6637 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.