Monomethyl auristatin E (MMAE)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
500mg	$260	In Stock
1g	$390	In Stock
5g	$1053	In Stock

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Cat #: V1887 CAS #: 474645-27-7 Purity ≥ 98%

Description: Monomethyl auristatin E (also known as MMAE; SGD-1010), a dolastatin 10 derivative, is a novel, synthetic and potent antimitotic/antitubulin agent which blocks the polymerization of tubulin, due to its high toxicity, it cannot be used as a drug itself; instead, it is linked to a monoclonal antibody (MAB) which directs it to the cancer cells.

References: Lisa Buckel, et al. Tumor radiosensitization by monomethyl auristatin E: mechanism of action and targeted delivery. Cancer Res. 2015 Apr 1;75(7):1376-87.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	717.98
Molecular Formula	C39H67N5O7
CAS No.	474645-27-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 100 mg/mL (139.3 mM)r
	Water: <1 mg/mLr
	Ethanol: 100 mg/mL (139.3 mM)
Synonyms	SGD1010; SGD 1010; SGD-1010; MMAE; Vedotin; Monomethyl auristatin E.

Protocol	In Vitro	MMAE sensitizes colorectal and pancreatic cancer cells to IR in a timeline and dose-dependent manner associated with mitotic arrest. Radiosensitization is manifested by decreased clonal viability and increased DNA double-strand breaks in irradiated cells.
Protocol	In Vivo	Monomethylauristatin E (MMAE) binds to IR to cause delayed tumor growth, and tumor-targeting ACPP-cRGD-MMAE with IR yields more robust and significantly prolonged tumor regression in xenograft models

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.3928 mL	6.9640 mL	13.9280 mL	27.8559 mL
5mM	0.2786 mL	1.3928 mL	2.7856 mL	5.5712 mL
10mM	0.1393 mL	0.6964 mL	1.3928 mL	2.7856 mL
20mM	0.0696 mL	0.3482 mL	0.6964 mL	1.3928 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.