ML204 HCl | CAS 2070015-10-8

ML204 HCl

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$450	Check With Us
500mg	$750	Check With Us
1g	$1125	Check With Us

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Cat #: V4005 CAS #: 2070015-10-8 Purity ≥ 98%

Description: ML204 HCl is a novel, potent, and selective TRPC4 (Transient receptor potential canonical) channel inhibitor identified from high throughput fluorescent screen of 305,000 compounds of the Molecular Libraries Small Molecule Repository for inhibitors that blocked intracellular Ca(2+) rise in response to stimulation of mouse TRPC4β by μ-opioid receptors.

References: Domingos M S Pereira, et al. Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice. Oxid Med Cell Longev. 2018 Jun 10;2018:4904696.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	262.78
Molecular Formula	C15H19ClN2
CAS No.	2070015-10-8
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 30mg/mLr
	Water: N/Ar
	Ethanol: N/A
SMILES Code	CC1=CC(N2CCCCC2)=NC3=CC=CC=C13.[H]Cl
Synonyms	ML204 HCl; ML-204; ML 204

Protocol	In Vivo	ML204 hydrochloride (1 mg/kg; s.c.; twice a day; for 5 days) causes mortality associated with exacerbated hypothermia and decreases peritoneal leukocyte numbers and cytokines in LPS-injected mice
	Animal model	Nonfasted male C57BL/6 (2-3 months)
	Dosages	1 mg/kg
	Administration	Subcutaneous injection, twice a day, for 5 days (prior to LPS injection)

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.8055 mL	19.0273 mL	38.0546 mL	76.1093 mL
5mM	0.7611 mL	3.8055 mL	7.6109 mL	15.2219 mL
10mM	0.3805 mL	1.9027 mL	3.8055 mL	7.6109 mL
20mM	0.1903 mL	0.9514 mL	1.9027 mL	3.8055 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.