ML204 | CAS 5465-86-1

ML204

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$450	In Stock
500mg	$750	In Stock
1g	$1125	In Stock

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Cat #: V4004 CAS #: 5465-86-1 Purity ≥ 98%

Description: ML204 is a novel, potent, and selective TRPC4 (Transient receptor potential canonical) channel inhibitor identified from high throughput fluorescent screen of 305,000 compounds of the Molecular Libraries Small Molecule Repository for inhibitors that blocked intracellular Ca(2+) rise in response to stimulation of mouse TRPC4β by μ-opioid receptors.

References: Miller M, et al. Identification of ML204, a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels. J Biol Chem. 2011 Sep 23;286(38):33436-46.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	226.32
Molecular Formula	C15H18N2
CAS No.	5465-86-1
Storage	-20℃ for 3 years in powder formrr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 30mg/mLrr
	Water: N/Arr
	Ethanol: N/A
SMILES Code	CC1=CC(N2CCCCC2)=NC3=CC=CC=C13
Synonyms	ML204; ML-204; ML 204

Protocol	In Vitro	ML204 inhibits TRPC4β-mediated intracellular Ca2+ Elevated, IC50 The value is 0.96 μM (HEK293 cells) and is 6-fold selective for muscarinic receptor-coupled TRPC19 channel activation

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.4185 mL	22.0926 mL	44.1852 mL	88.3704 mL
5mM	0.8837 mL	4.4185 mL	8.8370 mL	17.6741 mL
10mM	0.4419 mL	2.2093 mL	4.4185 mL	8.8370 mL
20mM	0.2209 mL	1.1046 mL	2.2093 mL	4.4185 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.