LM10 | CAS 1316695-35-8

LM10

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
50mg	$800	Check With Us
100mg	$1450	Check With Us
5g	$2175	Check With Us

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Cat #: V24280 CAS #: 1316695-35-8 Purity ≥ 99%

Description: LM10 is a novel, potent and selective inhibitor of tryptophan 2,3-dioxygenase (TDO) with IC50 values of 0.62 and 2 μM for human and mouse TDO, respectively. LM10 exhibits selectivity for TDO ver IDO, MAO-A, MAO-B, and a panel of receptors and transporters.

References: Pilotte L, et al. Reversal of tumoral immune resistance by inhibition of tryptophan 2,3-dioxygenase. Proc Natl Acad Sci U S A. 2012;109(7):2497-2502.

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Molecular Weight (MW)	229.21
Molecular Formula	C11H8FN5
CAS No.	1316695-35-8
SMILES Code	FC1=CC2=C(C=C1)C(/C=C/C3=NN=NN3)=CN2
Synonyms	LM10; LM 10; LM-10

Protocol	In Vivo	LM10 does not inhibit IDO and has a high solubility and bioavailability without obvious signs of toxicity. LM10 displays a good TDO inhibition (Ki = 5.6 μM) with a competitive inhibition profile. LM10 (160 mg/kg; p.o.) prevents the growth of TDO-expressing P815 tumor cells and promotes better rejection of control clone P815B cl1, which does not express TDO. The plasma concentration of LM10 after oral administration of 160 mg/kg/day is between 20 and 40 μg/mL (87-175 μM), a concentration about 40 times above the IC50 measured in the cellular assay performed with the physiological concentration of plasma tryptophan.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.3628 mL	21.8141 mL	43.6281 mL	87.2562 mL
5mM	0.8726 mL	4.3628 mL	8.7256 mL	17.4512 mL
10mM	0.4363 mL	2.1814 mL	4.3628 mL	8.7256 mL
20mM	0.2181 mL	1.0907 mL	2.1814 mL	4.3628 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.