Leupeptin Hemisulfate (NK381)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
500mg	$1050	In Stock
1g	$1700	In Stock
5g	$4305	In Stock

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Cat #: V0703 CAS #: 103476-89-7 Purity ≥ 98%

Description: Leupeptin Hemisulfate (NK-381; N-acetyl-L-leucyl-L-leucyl-L-argininal) is a naturally occurring, membrane-permeable, reversible and competitive inhibitor of the serine and cysteine proteases with potential antioxidant and anti-inflammatory activity.

References: Fu L, et al. Mechanism of Microbial Metabolite Leupeptin in the Treatment of COVID-19 by Traditional Chinese Medicine Herbs. mBio. 2021 Oct 26;12(5):e0222021.

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Physicochemical and Storage Information
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Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	475.59
Molecular Formula	C20H38N6O4.1/2H2SO4
CAS No.	103476-89-7
Storage	-20℃ for 3 years in powder formrr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 95 mg/mL (199.8 mM)rr
	Water: 95 mg/mL (199.8 mM)rr
	Ethanol: 95 mg/mL (199.8 mM)
SMILES Code	CC(C)C[C@@H](C(N)=O)N(C([C@H](CC(C)C)NC(C)=O)=O)[C@H](C=O)CCCNC(N)=N.CC(C)C[C@@H](C(N)=O)N(C([C@H](CC(C)C)NC(C)=O)=O)[C@H](C=O)CCCNC(N)=N.O=S(O)(O)=O
Synonyms	NK-381; NK 381; Leupeptin hemisulfate; NK381;

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.1027 mL	10.5133 mL	21.0265 mL	42.0530 mL
5mM	0.4205 mL	2.1027 mL	4.2053 mL	8.4106 mL
10mM	0.2103 mL	1.0513 mL	2.1027 mL	4.2053 mL
20mM	0.1051 mL	0.5257 mL	1.0513 mL	2.1027 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.