Leflunomide | CAS 75706-12-6

Leflunomide

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
2g	$400	Check With Us
5g	$700	Check With Us
10g	$1050	Check With Us

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Cat #: V0854 CAS #: 75706-12-6 Purity ≥ 98%

Description: Leflunomide (formerly also known as HWA486; Leflunomidum, trade/brand name Arava) is a potent pyrimidine synthesis inhibitor belonging to the DMARD (disease-modifying antirheumatic drug), it is used as an immunosuppressant agent for treating inflammatory conditions such as RA-rheumatoid arthritis.

References: Davis JP, et al. The immunosuppressive metabolite of leflunomide is a potent inhibitor of human dihydroorotate dehydrogenase. Biochemistry. 1996 Jan 30;35(4):1270-3.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	270.21
Molecular Formula	C12H9F3N2O2
CAS No.	75706-12-6
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 54 mg/mL (199.8 mM)r
	Water: <1 mg/mLr
	Ethanol: 54 mg/mL (199.8 mM)
SMILES Code	O=C(C1=C(C)ON=C1)NC2=CC=C(C(F)(F)F)C=C2
Synonyms	HWA486; HWA 486; Leflunomide, Arava, Leflunomidum, HWA-486

Protocol	In Vitro	Leflunomide is actually a prodrug that has been shown to inhibit proliferation of mononuclear and T-cells. Leflunomide is an inhibitor of several protein tyrosine kinases, with IC50 values between 30 mM and 100 mM in vitro cellular and enzymatic assays.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.7008 mL	18.5041 mL	37.0083 mL	74.0165 mL
5mM	0.7402 mL	3.7008 mL	7.4017 mL	14.8033 mL
10mM	0.3701 mL	1.8504 mL	3.7008 mL	7.4017 mL
20mM	0.1850 mL	0.9252 mL	1.8504 mL	3.7008 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.