EOAI3402143
This product is for research use only, not for human use. We do not sell to patients.
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| Size | Price | Stock |
|---|---|---|
| 50mg | $850 | To Be Confirmed |
| 100mg | $1450 | To Be Confirmed |
| 200mg | $2175 | To Be Confirmed |
: Cat #: V25341 CAS #: EOAI3402143 Purity ≥ 99%
Description: EOAI3402143 (EOAI 3402143; EOAI-3402143) is a novel and potent inhibitor of Usp9x, Usp24 and deubiquitinase (DUB) with potential anticancer activity.
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- Related Biological Data
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| Molecular Weight (MW) | 503.424 |
|---|---|
| Molecular Formula | C25H28Cl2N4O3 |
| CAS No. | EOAI3402143 |
| SMILES Code | O=C(NC(C1=CC=C(OCCN2CCOCC2)C=C1)CCC)/C(C#N)=C/C3=NC(Cl)=CC=C3Cl |
| Synonyms | EOAI3402143 |
| Protocol | In Vitro | EOAI3402143 retains potent Usp9x and Usp5 inhibitory activity. EOAI3402143 dose-dependently inhibits Usp9x and Usp24 activity, increases tumor cell apoptosis. Treatment of UM-2, UM-6, UM-16, and UM-76 with Usp9x inhibitor EOAI3402143 (G9) dose-dependently suppresses cell survival, while 600 nM of EOAI3402143 completely suppresses UM-2 3D colony growth when compared to untreated vehicle controls |
|---|---|---|
| In Vivo | To examine this potential, the effect of EOAI3402143 (G9) treatment is investigated on human MIAPACA2 tumor xenografts. Human MIAPACA2 cells are injected subcutaneously into NSG mice. Primary tumor development is monitored by caliper measurements, and once measurable, mice are separated into two groups and are treated with either vehicle control (PEG300/DMSO) or G9 at 15 mg/kg. Tumor growth, animal weight, behavior, and mobility are monitored during treatment. In parallel, murine 8041 tumors are also established and subjected to similar G9 treatment and tumor monitoring regimen as the human MIAPACA2 xenografts. Consistent with the in vitro findings, Usp9x inhibition results in the suppression of tumor growth in human tumor xenografts, but any significant effect on the growth of 8041 tumors xenografts is not observed, although the Usp9x activity is inhibited effectively by EOAI3402143 treatment in both human MIAPACA2 and mouse 8041 xenograft tumors |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.9864 mL | 9.9320 mL | 19.8640 mL | 39.7279 mL |
| 5mM | 0.3973 mL | 1.9864 mL | 3.9728 mL | 7.9456 mL |
| 10mM | 0.1986 mL | 0.9932 mL | 1.9864 mL | 3.9728 mL |
| 20mM | 0.0993 mL | 0.4966 mL | 0.9932 mL | 1.9864 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.