EN6
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $750 | Check With Us |
| 500mg | $1050 | Check With Us |
| 1g | $1575 | Check With Us |
: Cat #: V32198 CAS #: 1808714-73-9 Purity ≥ 99%
Description: EN6 is a small-molecule in vivo activator of autophagy that covalently targets cysteine 277 in the ATP6V1A subunit of the lysosomal the vacuolar H+ ATPase (v-ATPase).
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| Molecular Weight (MW) | 368.34 |
|---|---|
| Molecular Formula | C19H14F2N4O2 |
| CAS No. | 1808714-73-9 |
| SMILES Code | O=C(C1=CN(C2=CC=CC=C2F)N=C1)NC3=CC=C(F)C(NC(C=C)=O)=C3 |
| Synonyms | EN6; EN-6; EN 6 |
| Protocol | In Vitro | EN6 (25 μM; 7 h) promotes autophagic clearance of protein aggregates in IPTG-inducible GFP-TDP43 U2OS osteosarcoma cell line model. EN6 (50 μM; 4 h) activates the v-ATPase and lysosome acidification in HEK293A cells. EN6 (25 μM; 1 h) blocks mTORC1 lysosomal localization and activation in HEK293A cells. EN6 leads to significant increases in the number autophagosomes and autolysosomes in HEK293A cells. EN6 (50 μM; 1, 4, 8 h) increases the levels of LC3BII, and triggers formation of LC3 puncta in a time- and dose-dependent manner, in HEK293A cells. |
|---|---|---|
| In Vivo | EN6 (50 mg/kg; i.p.; single) inhibits mTORC1 and activates autophagy in vivo. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.7149 mL | 13.5744 mL | 27.1488 mL | 54.2977 mL |
| 5mM | 0.5430 mL | 2.7149 mL | 5.4298 mL | 10.8595 mL |
| 10mM | 0.2715 mL | 1.3574 mL | 2.7149 mL | 5.4298 mL |
| 20mM | 0.1357 mL | 0.6787 mL | 1.3574 mL | 2.7149 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.