Elaiophylin (Azalomycin B)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100mg	$3300	Check With Us
200mg	$4950	Check With Us
500mg	$8350	Check With Us

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Cat #: V2500 CAS #: 37318-06-2 Purity ≥ 98%

Description: Elaiophylin (also known as Azalomycin-B) shows antiprotozoal activity against Plasmodium falciparum K1a and Trypanosoma brucei brucei GUTat 3.1 strains with IC50 of 0.36 μM and 0.45 μM, respectively.

References: Zhao X, et al Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells. Autophagy. 2015;11(10):1849-63.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	1025.27
Molecular Formula	C54H88O18
CAS No.	37318-06-2
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 49 mg/mL (47.8 mM)r
	Water: <1 mg/mLr
	Ethanol: 5 mg/mL (4.9 mM)
SMILES Code	O[C@@H]1[C@H](O)[C@H](C)O[C@@]([H])(O[C@H]2[C@H](CC)[C@@H](C)O[C@@](O)([C@@H](C)[C@H](O)[C@H](C)[C@]([H])([C@@H](C)/C=C/C=C/3)OC(/C=C/C=C/[C@H](C)[C@@]([H])([C@@H](C)[C@@H](O)[C@H](C)[C@@]4(O)C[C@@H](O[C@@]5([H])C[C@H](O)[C@H](O)[C@H](C)O5)[C@H](CC)[C@@H](C)O4)OC3=O)=O)C2)C1
Synonyms	Azalomycin-B; Azalomycin B; Gopalamicin; Salbomycin

Protocol	In Vitro	Elaiophylin-mediated autophagy inhibition and lysosomal dysfunction affect ovarian cancer cell survival during hypoxia. Exposure to Elaiophylin (0.025-0.5 μM; 24 hours) causes a significant increase in ovarian cancer SKOV3 cell death in hypoxia conditions. In both the SKOV3 and A2780 cell lines, Elaiophylin (0.25, 0.5, 0.75 μM; 24 hours) treatment leads to significant activation of cleaved CASP9/caspase-9 and PARP1 and downregulation of BIRC5/survivin in a concentration-dependent manner.
Protocol	In Vivo	Treatment with 2 mg/kg Elaiophylin (given i.p. every 2 days for 21 days; in BALB/C athymic mice) significantly suppresses ovarian cancer SKOV3 cells growth compared with DMSO treatment, resulting in a 72% decrease in the average daily tumor growth rate compared with DMSO treatment . Lower doses of Elaiophylin as a single agent exert significant antitumor activity, while higher doses lead to intestinal toxicity. Administration of a lower dose (2 mg/kg) of Elaiophylin as a single agent achieves a significant antitumor effect without toxicity in an orthotopic ovarian cancer model with metastasis. Toxic reactions are observed only in the 8 mg/kg group.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	0.9754 mL	4.8768 mL	9.7535 mL	19.5071 mL
5mM	0.1951 mL	0.9754 mL	1.9507 mL	3.9014 mL
10mM	0.0975 mL	0.4877 mL	0.9754 mL	1.9507 mL
20mM	0.0488 mL	0.2438 mL	0.4877 mL	0.9754 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.