Dp44mT
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $450 | In Stock |
| 500mg | $750 | In Stock |
| 1g | $1125 | In Stock |
: Cat #: V1888 CAS #: 152095-12-0 Purity ≥ 98%
Description: Dp44mT is a novel and potent iron chelator with DNA-damaging activity mediated by top2a inhibition and with selective antitumor activity.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 285.37 |
|---|---|
| Molecular Formula | C14H15N5S |
| CAS No. | 152095-12-0 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 57 mg/mL (199.7 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: 47 mg/mL (164.7 mM) | |
| Solubility In Vivo | Propylene glycol: 1 mg/mL |
| SMILES Code | S=C(N/N=C(C1=NC=CC=C1)/C2=NC=CC=C2)N |
| Synonyms | Dp 44mt; 3-(Dipyridin-2-ylmethylideneamino)-1,1-dimethylthiourea |
| Protocol | In Vitro | Dp44mT is cytotoxic to breast cancer cells, at least in part, due to selective inhibition of top2α. Dp44mT alone induced selective cell killing in the breast cancer cell line MDA-MB-231 when compared with healthy mammary epithelial cells (MCF-12A). It induces G1 cell cycle arrest and reduces cancer cell clonogenic growth at nanomolar concentrations. Dp44mT, but not the iron chelator desferal, induces DNA double-strand breaks quantified as S139 phosphorylated histone foci (γ-H2AX) and Comet tails induced in MDA-MB-231 cells. Doxorubicin-induced cytotoxicity and DNA damage are both enhanced significantly in the presence of low concentrations of Dp44mT. The chelator caused selective poisoning of DNA topoisomerase IIα (top2α) as measured by an in vitro DNA cleavage assay and cellular topoisomerase-DNA complex formation. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.5042 mL | 17.5211 mL | 35.0422 mL | 70.0845 mL |
| 5mM | 0.7008 mL | 3.5042 mL | 7.0084 mL | 14.0169 mL |
| 10mM | 0.3504 mL | 1.7521 mL | 3.5042 mL | 7.0084 mL |
| 20mM | 0.1752 mL | 0.8761 mL | 1.7521 mL | 3.5042 mL |
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.