Doxofylline | CAS 69975-86-6

Doxofylline

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
5g	$380	Check With Us
10g	$650	Check With Us
20g	$975	Check With Us

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Cat #: V0794 CAS #: 69975-86-6 Purity ≥ 99%

Description: Doxofylline (also known as Neothylline; Ansimar; ABC-1213; ALT-07; Lufyllin; DO-309; Diprophylline; Corphyllin; ABC-12-3), a xanthine derivative, is a novel and potent PDE (phosphodiesterase) inhibitor with the potential for treating asthma.

References: Jiao P, et al. The protective effect of doxofylline against lipopolysaccharides (LPS)-induced activation of NLRP3 inflammasome is mediated by SIRT1 in human pulmonary bronchial epithelial cells. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):687-694.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	266.25
Molecular Formula	C11H14N4O4
CAS No.	69975-86-6
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 53 mg/mL (199.1 mM)r
	Water:<1 mg/mLr
	Ethanol: 53 mg/mL (199.1 mM)
SMILES Code	O=C(N1C)N(C)C2=C(N(CC3OCCO3)C=N2)C1=O
Synonyms	Doxofylline; Ansimar; ABC-1213; ALT-07; DO-309; Diprophylline; Lufyllin; Corphyllin; Neothylline; ABC 1213; ALT 07; DO 309; ABC1213; ALT07; DO309

Protocol	In Vitro	Doxofylline (5, 10 µM; 48 h) inhibits LPS-induced NLRP3 inflammasome activation and secretion of IL-1b and IL-18, as well as mitigates LPS-mediated SIRT1 reduction. Doxofylline (5, 10 µM; 48 h) suppresses LPS-induced expression of NADPH oxidase subunits and TXNIP 16HBE cells. Doxofylline (5, 10 µM; 48 h) shows potent protection against LPS-induced epithelial inflammation by reducing PGE2, NO release, and decreasing mitochondrial ROS generation in 16HBE cells.
Protocol	In Vivo	Doxofylline (0.3, 1 mg/kg; i.p.; single) inhibits LPS-induced inflammation in the lungs of mice.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.7559 mL	18.7793 mL	37.5587 mL	75.1174 mL
5mM	0.7512 mL	3.7559 mL	7.5117 mL	15.0235 mL
10mM	0.3756 mL	1.8779 mL	3.7559 mL	7.5117 mL
20mM	0.1878 mL	0.9390 mL	1.8779 mL	3.7559 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.