Disulfiram

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100g	$260	In Stock
200g	$400	In Stock
500g	$675	In Stock

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Cat #: V0859 CAS #: 97-77-8 Purity ≥ 98%

Description: Disulfiram (Disulfan; NSC 190940; Dicupral; Disetil; NSC190940; Tetraethylthiuram disulfide; TETD) is a potent and specific inhibitor of aldehyde-dehydrogenase (ALDH1) that is used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol.

References: Chen D, ert al. Disulfiram, a clinically used anti-alcoholism drug and copper-binding agent, induces apoptotic cell death in breast cancer cultures and?xenografts?via?inhibition?of the proteasome?activity. Cancer Res. 2006 Nov 1;66(21):10425-33.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	296.54
Molecular Formula	C10H20N2S4
CAS No.	97-77-8
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 59 mg/mL (199.0 mM)r
	Water: <1 mg/mLr
	Ethanol: 59 mg/mL (199.0 mM)
SMILES Code	S=C(SSC(N(CC)CC)=S)N(CC)CC
Synonyms	Disulfan; NSC 190940; Dicupral; Disetil; NSC190940; NSC-190940; Disulfiram;

Protocol	In Vitro	Disulfiram-copper complex potently inhibits the proteasomal activity in cultured breast cancer MDA-MB-231 and MCF10DCIS.com cells, but not normal, immortalized MCF-10A cells, before induction of apoptotic cancer cell death. Disulfiram (0.1 nM-10 μM; 72 h)?+?Cu2+ combination enhances the cytotoxicity on ovarian cancer cell lines.
Protocol	In Vivo	Disulfiram significantly inhibits the tumor growth (by 74%), associated with in vivo proteasome inhibition and apoptosis induction in mice bearing MDA-MB-231 tumor xenograf. Apoptosis is shown by caspase activation and apoptotic nuclei formation. Proteasome inhibition is measured by decreased levels of tumor tissue proteasome activity and accumulation of ubiquitinated proteins and natural proteasome substrates p27 and Bax.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.3722 mL	16.8611 mL	33.7223 mL	67.4445 mL
5mM	0.6744 mL	3.3722 mL	6.7445 mL	13.4889 mL
10mM	0.3372 mL	1.6861 mL	3.3722 mL	6.7445 mL
20mM	0.1686 mL	0.8431 mL	1.6861 mL	3.3722 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.