Digoxin (12β-Hydroxydigitoxin)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
1g	$150	Check With Us
10g	$650	Check With Us
20g	$975	Check With Us

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Cat #: V1648 CAS #: 20830-75-5 Purity ≥ 98%

Description: Digoxin (12β-Hydroxydigitoxin; Dixina; HSDB-214; Novodigal; Lanoxin PG; SK-Digoxin; Cardiogoxin) is a sodium-potassium pump inhibitor and a cardiotonic glycoside that has been approved for use in the treatment of various heart conditions such as arrhythmia and heart failure.

References: Ashbrook AW, et al. Antagonism of the Sodium-Potassium ATPase Impairs Chikungunya Virus Infection. MBio. 2016 May 24; 7(3). pii: e00693-16.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	780.94
Molecular Formula	C41H64O14
CAS No.	20830-75-5
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 100 mg/mL (128.1 mM)r
	Water:<1 mg/mLr
	Ethanol:<1 mg/mL
Synonyms	HSDB214; HSDB 214; 12β-Hydroxydigitoxin; Digoxin; Dixina; HSDB-214; Novodigal; Lanoxin PG; SK-Digoxin; SK Digoxin; Cardiogoxin;

Protocol	In Vitro	Digoxin is a sodium-potassium ATPase inhibitor with a negative Z-score of -26.67, indicating that sodium-potassium ATPase plays a role in CHIKV infection. Digoxin-treated U-2 OS cells resulted in a dose-dependent reduction in CHIKV infection relative to DMSO-treated cells, with half the maximum effective concentration (EC).50) is 48.8 nM. Digoxin treatment similarly reduced CHIKV infection in primary human synovial fibroblasts (HSF) and Vero African green monkey kidney cells, EC50 43.9 nM and 67.3 nM, respectively. Digoxin treatment significantly reduces CHIKV infection in these cell types, EC in ST2 cells50 EC in 16.2 μM, C2C12 cells50 is 23.2 μM, which is the EC50 330 and 475 times Digoxin in U-2 OS cells. Cell viability was only slightly impaired after 1 h treatment with 24 μM Digoxin, the dose of CHIKV antiviral activity in these cells Digoxin EC50 20 times

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.2805 mL	6.4025 mL	12.8051 mL	25.6102 mL
5mM	0.2561 mL	1.2805 mL	2.5610 mL	5.1220 mL
10mM	0.1281 mL	0.6403 mL	1.2805 mL	2.5610 mL
20mM	0.0640 mL	0.3201 mL	0.6403 mL	1.2805 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.