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Daphnetin (7,8-dihydroxycoumarin)

This product is for research use only, not for human use. We do not sell to patients.

Daphnetin (7,8-dihydroxycoumarin)
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
1g$250Check With Us
5g$750Check With Us
10g$1125Check With Us
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Cat #: V1476 CAS #: 486-35-1 Purity ≥ 98%

Description: Daphnetin (NSC-633563; NSC633563; 7,8-dihydroxycoumarin), a naturally occuring coumarin analog isolated from plants of the genus Daphne, is a PKI-protein kinase inhibitor with important biological activity (e.g. anti-inflammatory and anti-oxidant effects).

References: Fan X, et, al. Daphnetin triggers ROS-induced cell death and induces cytoprotective autophagy by modulating the AMPK/Akt/mTOR pathway in ovarian cancer. Phytomedicine. 2021 Feb;82:153465.

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Molecular Weight (MW)178.14
Molecular FormulaC9H6O4
CAS No.486-35-1
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 35 mg/mL (196.5 mM)r
Water: <1 mg/mLr
Ethanol: <1 mg/mL
SynonymsNSC 633563; 7,8-Dihydroxycoumarin; NSC-633563; NSC633563;
ProtocolIn VitroDaphnetin (7,8-dihydroxycoumarin) (0-40 µg/mL; 24-48 hours) inhibits the proliferation of ovarian cancer cells
In VivoDaphnetin (7,8-dihydroxycoumarin) (30 mg/kg; i.p.; daily; for 12 days; BALB/c nude mice) has antitumour activities in a xenograft animal model
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM5.6136 mL28.0678 mL56.1356 mL112.2712 mL
5mM1.1227 mL5.6136 mL11.2271 mL22.4542 mL
10mM0.5614 mL2.8068 mL5.6136 mL11.2271 mL
20mM0.2807 mL1.4034 mL2.8068 mL5.6136 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
 
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The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
 
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Volume(start)
V1
 
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Concentration(final)
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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.