D-64131
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $850 | Check With Us |
| 500mg | $1300 | Check With Us |
| 1g | $1950 | Check With Us |
: Cat #: V2890 CAS #: 74588-78-6 Purity ≥ 98%
Description: D-64131, an aroylindole analog, is a novel, oral and potent inhibitor of tubulin polymerization. As a mitotic inhibitor, D-64131 competitively binds to the colchicine binding site of αβ-Tubulin.
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- Physicochemical and Storage Information
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- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 251.28 |
|---|---|
| Molecular Formula | C16H13NO2 |
| CAS No. | 74588-78-6 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 10 mMr |
| Water: <1 mg/mLr | |
| Ethanol: <1 mg/mL | |
| SMILES Code | O=C(C(N1)=CC2=C1C=CC(OC)=C2)C3=CC=CC=C3 |
| Synonyms | D-64131; D 64131; D64131; (5-Methoxy-1H-indol-2-yl)phenylmethanone |
| Protocol | In Vitro | D-64131 is antimitotic by binding to β-tubulin, thereby destabilizing microtubules and arresting mitotic cells in the M-phase[1]. D-64131 inhibits the proliferation of tumor cells from 12 of 14 different organs and tissues with mean IC50s of 62 nM[1]. D-64131 is cytotoxic toward MDR/MRP tumor cell lines |
|---|---|---|
| In Vivo | D-64131 (200-400 mg/kg; p.o.; daily; days 1-5, 8-9, and 15-18) significantly inhibits tumor growth in the human amelanoic melanoma MEXF 989 tumor xenograft mice model | |
| Animal model | Outbred nude mice (6-8 weeks), human amelanoic melanoma MEXF 989 tumor xenograft model | |
| Dosages | 200 mg/kg, 400 mg/kg | |
| Administration | Oral administration, daily, on days 1-5, 8-9, and 15-18 after xenograft |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.9796 mL | 19.8981 mL | 39.7962 mL | 79.5925 mL |
| 5mM | 0.7959 mL | 3.9796 mL | 7.9592 mL | 15.9185 mL |
| 10mM | 0.3980 mL | 1.9898 mL | 3.9796 mL | 7.9592 mL |
| 20mM | 0.1990 mL | 0.9949 mL | 1.9898 mL | 3.9796 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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Volume
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The dilution calculator equation
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Volume(start)
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This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
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V1
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C2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.