Cisplatin (CDDP)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
2g	$220	In Stock
5g	$400	In Stock
10g	$600	In Stock

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Cat #: V1327 CAS #: 15663-27-1 Purity ≥ 98%

Description: Cisplatin (CDDP; cis-Diaminodichloroplatinum; Trade names: Platinol; PlatinolAQ among others), an inorganic platinum complex acting as a DNA intercalating agent, is a widely used and classic chemotherapeutic drug and a potent inducer of growth arrest and apoptosis in a variety of cell types.

References: Wang X, et al. Requirement for ERK activation in cisplatin-induced apoptosis. J Biol Chem. 2000 Dec 15;275(50):39435-43.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	300.05
Molecular Formula	Cl2H6N2Pt
CAS No.	15663-27-1
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: >10 mLr
	Water: <1 mg/mLr
	Ethanol:<1 mg/mL (slightly soluble or insoluble)
Solubility In Vivo	Saline: 3 mg/mL
SMILES Code	Cl[Pt-2]([NH3+])([NH3+])Cl
Synonyms	Cismaplat; Cisplatina; cisplatinous diamine dichloride; cisplatinum; cisplatinum II; cisplatinum II diamine dichloride; CDDP; DDP; cisDDP; cisdiamminedichloro platinum (II); cisdiamminedichloroplatinum; Cisdichloroammine Platinum (II); CPDD; Cysplatyna; DDP; PDD; Peyrones Chloride; Peyrones Salt; CACP; Platinoxan; platinum diamminodichloride. Trade names (US): Platinol; PlatinolAQ. Trade names (other countries): Abiplatin; Blastolem; Briplatin; Cisplatyl; Citoplatino; Citosin; Lederplatin; Metaplatin; Neoplatin; Placis; Platamine; Platiblastin; PlatiblastinS; Platinex; Platinol AQ; PlatinolAQ VHA Plus; Platiran; Platistin; Platosin. Chemical Name: (SP-4-2)-diamminedichloroplatinum; platinum, diaminedichloro-, cis-

Protocol	In Vitro	Cisplatin (CDDP) causes apoptosis of HeLa cells in a dose-dependent manner, with a concentration of 30 μM Cisplatin resulting in death of greater than 90% of the cell population by 24 h of treatment. The kinetics of Cisplatin-induced apoptosis are examined using a 30 μM concentration. Cisplatin Activates the MEK/ERK Signaling Pathway, 20 and 30 μM Cisplatin, both of which results in significant apoptosis, leads to strong activation of ERK

These protocols are for reference only. InvivoChem does not independently validate these methods.

Related Biological Data

The inhibition of tumor growth was enhanced by HemoHIM administration in melanoma-bearing mice which were injected with cisplatin. BMC Cancer. 2009 Mar 17;9:85.

Growth inhibition effect of cisplatin and HemoHIM on melanoma cells in vitro. BMC Cancer. 2009 Mar 17;9:85.

HemoHIM administration promotes immune responses for tumor surveillance in melanoma-bearing mice which were injected with cisplatin. BMC Cancer. 2009 Mar 17;9:85.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.3328 mL	16.6639 mL	33.3278 mL	66.6556 mL
5mM	0.6666 mL	3.3328 mL	6.6656 mL	13.3311 mL
10mM	0.3333 mL	1.6664 mL	3.3328 mL	6.6656 mL
20mM	0.1666 mL	0.8332 mL	1.6664 mL	3.3328 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.