Cholic Acid

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100g	$150	Check With Us
200g	$250	Check With Us
500g	$420	Check With Us

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Cat #: V9127 CAS #: 81-25-4 Purity ≥ 98%

Description: Cholic Acid (E-1000; NSC-6135; Cholalin; Orphacol; Cholbam) is a bile acid that has been approved in 2015 for use in the treatment of symptoms of bile acid synthesis disorders, peroxisomal disorders.

References: Li Y, et al. Mechanism of hepatic targeting via oral administration of DSPE-PEG-Cholic acid-modified nanoliposomes. Int J Nanomedicine. 2017 Feb 28;12:1673-1684.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	408.57
Molecular Formula	C24H40O5
CAS No.	81-25-4
SMILES Code	C[C@@]12[C@@H](C(C)CCC(O)=O)CC[C@@]1([H])[C@]3([H])[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@@]3([H])C[C@@H]2O
Synonyms	Cholic Acid; E-1000; NSC-6135; E-1000; NSC-6135; Orphacol; Cholalin; Cholalic acid; Colalin; E-1000; NSC-6135;

Protocol	In Vitro	Cholic acid (1 mg/mL, 30 min) competitively binds Na+/taurocholate cotransporting polypeptide (NTCP) on HepG2 cells and significantly inhibits the uptake of Cholic acid (CA)-nanoliposomes (LPs)-Doxorubicin (DOX)-HCl, which indicates that CA-LPs-DOX-HCl are also uptaken via NTCP-mediated endocytosis pathway.
Protocol	In Vivo	Cholic acid (1% (w/w) Cholic acid-supplemented diet; p.o.; 14 days) decreases SHP (small heterodimer partner) protein expression, potentially via the upregulation of miR142-3p. Cholic acid increases CYP2D6 expression and activity.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.4476 mL	12.2378 mL	24.4756 mL	48.9512 mL
5mM	0.4895 mL	2.4476 mL	4.8951 mL	9.7902 mL
10mM	0.2448 mL	1.2238 mL	2.4476 mL	4.8951 mL
20mM	0.1224 mL	0.6119 mL	1.2238 mL	2.4476 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.