BTB-1
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 1g | $520 | Check With Us |
| 5g | $780 | Check With Us |
| 10g | $1170 | Check With Us |
: Cat #: V3229 CAS #: 86030-08-2 Purity ≥ 98%
Description: BTB-1 is a novel, potent and the first small molecule inhibitor of the mitotic motor protein Kif18A.
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| Molecular Weight (MW) | 297.71 |
|---|---|
| Molecular Formula | C12H8ClNO4S |
| CAS No. | 86030-08-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: ≥ 100 mg/mLr |
| Water: N/Ar | |
| Ethanol: N/A | |
| SMILES Code | O=S(C1=CC=C(Cl)C=C1[N+]([O-])=O)(C2=CC=CC=C2)=O |
| Synonyms | BTB-1; BTB 1; BTB1. |
| Protocol | In Vitro | BTB-1 blocks the motility of Kif18A in a reversible manner. BTB-1 inhibits Kif18A in an adenosine triphosphate (ATP)-competitive but microtubule-uncompetitive manner and slows down the progression of cells through mitosis. 100 μM BTB-1 does not significantly inhibit any of the other tested mitotic kinesins. BTB-1 competes with ATP for Kif18A binding only when the motor-protein is associated with its pseudosubstrate microtubules. HeLa cells treated with BTB-1 accumulate in mitosis in a dose-dependent manner. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.3590 mL | 16.7949 mL | 33.5897 mL | 67.1795 mL |
| 5mM | 0.6718 mL | 3.3590 mL | 6.7179 mL | 13.4359 mL |
| 10mM | 0.3359 mL | 1.6795 mL | 3.3590 mL | 6.7179 mL |
| 20mM | 0.1679 mL | 0.8397 mL | 1.6795 mL | 3.3590 mL |
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.