Biocytin
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $185 | In Stock |
| 500mg | $260 | In Stock |
| 1g | $390 | In Stock |
: Cat #: V5469 CAS #: 576-19-2 Purity ≥ 98%
Description: Biocytin is a conjugated adduct of L-lysine and D-biotin.
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| Molecular Weight (MW) | 372.48 |
|---|---|
| Molecular Formula | C16H28N4O4S |
| CAS No. | 576-19-2 |
| SMILES Code | N[C@@H](CCCCNC(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(O)=O |
| Synonyms | Biocytin; Biotinyl-L-lysine; |
| Protocol | In Vitro | Due to its high affinity for avidin, Biocytin can be visualized by using a host of avidin-conjugated markers at the light and electron microscopic level. Biocytin is used to visualize the morphology of dendritic and axonal arborizations. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.6847 mL | 13.4235 mL | 26.8471 mL | 53.6942 mL |
| 5mM | 0.5369 mL | 2.6847 mL | 5.3694 mL | 10.7388 mL |
| 10mM | 0.2685 mL | 1.3424 mL | 2.6847 mL | 5.3694 mL |
| 20mM | 0.1342 mL | 0.6712 mL | 1.3424 mL | 2.6847 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.