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A-317567

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A-317567
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Cat #: V3091 CAS #: 371217-32-2 Purity ≥ 98%

Description: A-317567 (A317567) is a novel non-amiloride inhibitor of the acid-sensing ion channel (ASIC) (IC50 = 1.025 μM) with antidepressant and antinociception effects. It acts on three distinct types of native ASIC currents evoked in acutely dissociated adult rat dorsal root ganglion (DRG) neurons. A-317567 produced concentration-dependent inhibition of all pH 4.5-evoked ASIC currents with an IC50 ranging between 2 and 30muM, depending upon the type of ASIC current activated. Unlike amiloride, A-317567 equipotently blocked the sustained phase of ASIC3-like current, a biphasic current akin to cloned ASIC3, which is predominant in DRG. When evaluated in the rat Complete Freud's Adjuvant (CFA)-induced inflammatory thermal hyperalgesia model, A-317567 was fully efficacious at a dose 10-fold lower than amiloride.

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Molecular Weight (MW)C27H31N3
Molecular Formula397.57
CAS No.371217-32-2
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 10 mM
Water: N/A
Ethanol: N/A
SynonymsA317567; A 317567; A-317567
ProtocolIn VitroThe effects of A-317567 (A317567) on three distinct types of native ASIC currents evoked in acutely dissociated adult rat dorsal root ganglion (DRG) neurons are studied. A-317567 produces concentration-dependent inhibition of all pH 4.5-evoked ASIC currents with an IC50 ranging between 2 and 30 μM, depending upon the type of ASIC current activated. A-317567 equipotently blocks the sustained phase of ASIC3-like current, a biphasic current akin to cloned ASIC3, which is predominant in DRG[2].
In VivoA-317567 (A317567) is fully analgesic effects in the CFA model (ED50 of 17 μmol/kg, i.p., adult male Sprague-Dawley rats). There is no significant effect of A-317567 on the withdrawal latency of the contralateral paw under these conditions[2].
These protocols are for reference only. InvivoChem does not independently validate these methods.
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