A 274

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A 274
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Cat #: V3079 CAS #: 77273-75-7 Purity ≥ 98%

Description: A274 is an extensively conjugated phenol that has recently shown to be present as a common trace constituent in randomly selected batches of commercial tissue culture media. It is a highly aromatic and mono-substituted phenol antioxidant produced during differentiation of neuroblastoma cells.

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Molecular Weight (MW)274.32
Molecular FormulaC19H14O2
CAS No.77273-75-7
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility In VitroDMSO:
Water:
Ethanol:
SynonymsA 274; A274; A-274
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.6454 mL18.2269 mL36.4538 mL72.9076 mL
5mM0.7291 mL3.6454 mL7.2908 mL14.5815 mL
10mM0.3645 mL1.8227 mL3.6454 mL7.2908 mL
20mM0.1823 mL0.9113 mL1.8227 mL3.6454 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.