A 274 | CAS 77273-75-7

A 274

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Cat #: V3079 CAS #: 77273-75-7 Purity ≥ 98%

Description: A274 is an extensively conjugated phenol that has recently shown to be present as a common trace constituent in randomly selected batches of commercial tissue culture media. It is a highly aromatic and mono-substituted phenol antioxidant produced during differentiation of neuroblastoma cells.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	274.32
Molecular Formula	C19H14O2
CAS No.	77273-75-7
Storage	-20℃ for 3 years in powder form
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO:
	Water:
	Ethanol:
Synonyms	A 274; A274; A-274

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.6454 mL	18.2269 mL	36.4538 mL	72.9076 mL
5mM	0.7291 mL	3.6454 mL	7.2908 mL	14.5815 mL
10mM	0.3645 mL	1.8227 mL	3.6454 mL	7.2908 mL
20mM	0.1823 mL	0.9113 mL	1.8227 mL	3.6454 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.