Benzamil

This product is for research use only, not for human use. We do not sell to patients.

Benzamil
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
100mg$400Check With Us
250mg$750Check With Us
500mg$1125Check With Us

Cat #: V37989 CAS #: 2898-76-2 Purity ≥ 98%

Description: Benzamil is an Amiloride analogue acting as a Na+/Ca2+ exchanger (NCX) inhibitor (IC50~100 nM) with the potential to treat cystic fibrosis.

References: Castañeda MS, et al. Benzamil inhibits neuronal and heterologously expressed small conductance Ca2+-activated K+channels. Neuropharmacology. 2019 Nov 1;158:107738.

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Promise
Molecular Weight (MW)319.75
Molecular FormulaC₁₃H₁₄ClN₇O
CAS No.2898-76-2
SynonymsBenzamil
ProtocolIn VitroBenzamil (Benzylamiloride) inhibits neuronal and heterologously expressed small conductance Ca2+-activated K2+ channels
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.1274 mL15.6372 mL31.2744 mL62.5489 mL
5mM0.6255 mL3.1274 mL6.2549 mL12.5098 mL
10mM0.3127 mL1.5637 mL3.1274 mL6.2549 mL
20mM0.1564 mL0.7819 mL1.5637 mL3.1274 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.