BEC HCl
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $950 | Check With Us |
| 1g | $1650 | Check With Us |
| 5g | $4175 | Check With Us |
: Cat #: V5730 CAS #: 222638-67-7 Purity ≥ 99%
Description: BEC HCl is a novel and potent Arginase II inhibitor (Ki = 0.31 μM), the binding is slow and competitive.
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| Molecular Weight (MW) | 229.49 |
|---|---|
| Molecular Formula | C5H13BClNO4S |
| CAS No. | 222638-67-7 |
| SMILES Code | N[C@@H](CSCCB(O)O)C(O)=O.[H]Cl |
| Synonyms | BEC HCl; S-(2-boronoethyl)-L-cysteine |
| Protocol | In Vitro | The X-ray crystal structure of the arginase-BEC complex has been determined at 2.3 Å resolution from crystals perfectly twinned by hemihedry. The structure of the complex reveals that the boronic acid moiety undergoes nucleophilic attack by metal-bridging hydroxide ion to yield a tetrahedral boronate anion that bridges the binuclear manganese cluster, thereby mimicking the tetrahedral intermediate (and its flanking transition states) in the arginine hydrolysis reaction. |
|---|---|---|
| In Vivo | Administration of the arginase inhibitor BEC decreases arginase activity and causes alterations in NO homeostasis, which are reflected by increases in S-nitrosylated and nitrated proteins in the lungs from inflamed mice. BEC enhances perivascular and peribronchiolar lung inflammation, mucus metaplasia, NF-κB DNA binding, and mRNA expression of the NF-κB-driven chemokine genes CCL20 and KC, and leads to further increases in airways hyperresponsiveness. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 4.3575 mL | 21.7874 mL | 43.5749 mL | 87.1498 mL |
| 5mM | 0.8715 mL | 4.3575 mL | 8.7150 mL | 17.4300 mL |
| 10mM | 0.4357 mL | 2.1787 mL | 4.3575 mL | 8.7150 mL |
| 20mM | 0.2179 mL | 1.0894 mL | 2.1787 mL | 4.3575 mL |
The molarity calculator equation
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.