Brefeldin A (BFA)

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
1g	$1250	Check With Us
2g	$2000	Check With Us
5g	$3375	Check With Us

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Cat #: V0160 CAS #: 20350-15-6 Purity ≥ 98%

Description: Brefeldin A (also known as BFA), a fungal metabolite, is a potent macrocyclic lactone antibiotic and ATPase inhibitor for intracellular vesicle formation and protein transport (protein trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus) with IC50 of 0.2 μM in HCT 116 cells.

References: Alvarez C, et al. Brefeldin A (BFA) disrupts the organization of the microtubule and the actin cytoskeletons. Eur J Cell Biol. 1999 Jan;78(1):1-14.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	280.36
Molecular Formula	C16H24O4
CAS No.	20350-15-6
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 4 mg/mL (14.3 mM)r
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
SMILES Code	O=C1O[C@@H](C)CCC/C=C/[C@]2([H])C[C@H](O)C[C@@]2([H])[C@H](O)/C=C/1
Synonyms	Brefeldin A; BFA; Cyanein; Decumbin; Brefeldin-A; Ascotoxin; Cyanein; Decumbin; Bredfeldin A; Synergisidin

Protocol	In Vitro	Brefeldin A (BFA) treatment for 15 h or 40 h, causes dramatic swelling of the Endoplasmic Reticulum (ER) and shifts its localization to the periphery of normal rat kidney (NRK) cells. Prolonged Brefeldin A treatment results in marked disruption of the MT and actin cytoskeleton.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.5668 mL	17.8342 mL	35.6684 mL	71.3369 mL
5mM	0.7134 mL	3.5668 mL	7.1337 mL	14.2674 mL
10mM	0.3567 mL	1.7834 mL	3.5668 mL	7.1337 mL
20mM	0.1783 mL	0.8917 mL	1.7834 mL	3.5668 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.