CA-5F
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $750 | To Be Confirmed |
| 250mg | $1350 | To Be Confirmed |
| 500mg | $2025 | To Be Confirmed |
: Cat #: V3993 CAS #: 1370032-19-1 Purity ≥ 98%
Description: CA-5f (CA5f) is a novel and potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion with anti-tumor activity.
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- Related Biological Data
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| Molecular Weight (MW) | 388.46 |
|---|---|
| Molecular Formula | C₂₄H₂₄N₂O₃ |
| CAS No. | 1370032-19-1 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: ≥ 10 mMr |
| Water: N/Ar | |
| Ethanol: N/A | |
| SMILES Code | O=C(/C(CN(C)C/1)=C/C2=CNC3=C2C=CC=C3)C1=CC4=CC=C(OC)C(OC)=C4 |
| Synonyms | CA-5f; CA5f; CA 5f; |
| Protocol | In Vitro | CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs. CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes. CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs. CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs. |
|---|---|---|
| In Vivo | CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells. CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.5743 mL | 12.8713 mL | 25.7427 mL | 51.4854 mL |
| 5mM | 0.5149 mL | 2.5743 mL | 5.1485 mL | 10.2971 mL |
| 10mM | 0.2574 mL | 1.2871 mL | 2.5743 mL | 5.1485 mL |
| 20mM | 0.1287 mL | 0.6436 mL | 1.2871 mL | 2.5743 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
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Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.