Buformin
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $750 | To Be Confirmed |
| 1g | $1250 | To Be Confirmed |
| 5g | $3165 | To Be Confirmed |
: Cat #: V11178 CAS #: 692-13-7 Purity ≥ 99%
Description: Buformin (NSC-528218; 1-butylbiguanide) is an orally bioavailable AMPK activator approved for use as an antidiabetic drug.
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- Related Biological Data
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| Molecular Weight (MW) | 157.22 |
|---|---|
| Molecular Formula | C6H15N5 |
| CAS No. | 692-13-7 |
| SMILES Code | N=C(NC(N)=N)NCCCC |
| Synonyms | Buformin; H 224; W-37; W37; W 37; |
| Protocol | In Vitro | Buformin (0-3 mM; 24 hours) suppresses RTK activation, including erbB-2 and IGF1R signaling downstream, and Akt activation/phosphorylation is inhibited in both SKBR3 and BT474 cells. Buformin (0-3 mM; 48 hours) increases the percentage of cells in G0/G1 phase and reduced the percentage of cells in S phase, especially in the SKBR3 cells. Buformin (0-10 mM; 5 days) inhibits SKBR3 and BT474 cells growth as a concentration-dependent manner, exhibits IC50 values of 246.7 μM and 98.6 μM for erbB-2-overexpressing SKBR3 and BT474 cells, respectively. |
|---|---|---|
| In Vivo | Buformin (oral administation; 7.6 mmol/kg of chow; 7 days) exhibits significantly reduced tumor volumes and weights, and hinders mammary morphogenesis and proliferation in MMTV-erbB-2 transgenic mice. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 6.3605 mL | 31.8026 mL | 63.6051 mL | 127.2103 mL |
| 5mM | 1.2721 mL | 6.3605 mL | 12.7210 mL | 25.4421 mL |
| 10mM | 0.6361 mL | 3.1803 mL | 6.3605 mL | 12.7210 mL |
| 20mM | 0.3180 mL | 1.5901 mL | 3.1803 mL | 6.3605 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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Volume
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Molecular Weight*
The dilution calculator equation
Concentration(start)
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Volume(start)
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Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
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V1
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.