SB415286 | CAS 264218-23-7

SB415286

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
500mg	$1100	Check With Us
1g	$1700	Check With Us
5g	$4305	Check With Us

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Cat #: V0216 CAS #: 264218-23-7 Purity ≥ 98%

Description: SB415286 is a novel, potent, selective, cell permeable and ATP-competitive GSK3α (glycogen synthase kinase-3alpha) inhibitor with potential anti-inflammatory activity.

References: SB 415286 (SB-415286) inhibits human GSK-3α with an IC₅₀ of 77.5 nM, and a K_i of 30.75 nM. SB-415286 stimulates glycogen synthesis in the Chang human liver cell line with EC₅₀ of 2.9 μM. SB-415286 stimulates glycogen synthase activity in Chang human liver cells. SB-415286 induces transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	359.72
Molecular Formula	C16H10ClN3O5
CAS No.	264218-23-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 72 mg/mL (200.2 mM)r
	Water: <1 mg/mLr
	Ethanol: 72 mg/mL (200.2 mM)
SMILES Code	O=C(C(NC1=CC=C(O)C(Cl)=C1)=C2C3=CC=CC=C3[N+]([O-])=O)NC2=O
Synonyms	SB-415286; SB 415286; SB415286

Protocol	In Vitro	SB 415286 (SB-415286) inhibits human GSK-3α with an IC50 of 77.5 nM, and a Ki of 30.75 nM. SB-415286 stimulates glycogen synthesis in the Chang human liver cell line with EC50 of 2.9 μM. SB-415286 stimulates glycogen synthase activity in Chang human liver cells. SB-415286 induces transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.7799 mL	13.8997 mL	27.7994 mL	55.5988 mL
5mM	0.5560 mL	2.7799 mL	5.5599 mL	11.1198 mL
10mM	0.2780 mL	1.3900 mL	2.7799 mL	5.5599 mL
20mM	0.1390 mL	0.6950 mL	1.3900 mL	2.7799 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.