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| Size | Price | Stock |
|---|---|---|
| 100mg | $700 | Check With Us |
| 250mg | $1350 | Check With Us |
| 500mg | $2025 | Check With Us |
: Cat #: V0188 CAS #: 1139889-93-2 Purity ≥ 98%
Description: Golgicide A (GCA), a quinoline compound, is a cell-permeable, potent and rapidly reversible inhibitor of GBF1, which is one of the guanine nucleotide exchange factors (ArfGEFs). It is also an inhibitor of shiga toxin cytotoxicity. Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM in Vero cells. Immunofluoresence experiments demonstrates that Golgicide A causes complete dispersal of the medial-Golgi markers giantin and the cis-Golgi marker GM130 and results in a rapid redistribution of COPI from the Golgi.
References: [1]. Sáenz JB, et al. Golgicide A reveals essential roles for GBF1 in Golgi assembly and function. Nat Chem Biol. 2009 Mar;5(3):157-65.
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| Molecular Weight (MW) | 284.3 |
|---|---|
| Molecular Formula | C17H14F2N2 |
| CAS No. | 1139889-93-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 57 mg/mL (200.5 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: 4 mg/mL (14.1 mM) | |
| SMILES Code | FC1=CC2=C(NC(C3=CC=CN=C3)[C@@]4([H])[C@]2([H])C=CC4)C(F |
| Synonyms | Golgicide A; GCA |
| Protocol | In Vitro | In vitro activity: Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM. Golgicide A causes a decrease in GBF1-mediated Arf1 activation, arrests secretion of soluble and membrane-anchored proteins and then impairs retrograde toxin transport. Golgicide A decreases HCV RNA levels in FLRP1 cells and J6/JFH1 cells. In addition, Golgicide A causes redistribution of NS5A and accumulation of infectious viral particles in J6/JFH1 cells. Cell Assay: Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM in Vero cells. Immunofluoresence experiments demonstrates that Golgicide A causes complete dispersal of the medial-Golgi markers giantin and the cis-Golgi marker GM130 and results in a rapid redistribution of COPI from the Golgi. Also, Golgicide A causes a decrease in GBF1-mediated Arf1 activation, impairs retrograde toxin transport and arrests secretion of soluble and membrane-anchored proteins. Golgicide A decreases HCV RNA levels and causes redistribution of NS5A in FLRP1 cells and J6/JFH1 cells. In addition, Golgicide A causes accumulation of infectious viral particles in J6/JFH1 cells. |
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| Related Biological Data |
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| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.5174 mL | 17.5871 mL | 35.1741 mL | 70.3482 mL |
| 5mM | 0.7035 mL | 3.5174 mL | 7.0348 mL | 14.0696 mL |
| 10mM | 0.3517 mL | 1.7587 mL | 3.5174 mL | 7.0348 mL |
| 20mM | 0.1759 mL | 0.8794 mL | 1.7587 mL | 3.5174 mL |
This equation is commonly abbreviated as: C1 V1 = C2 V2
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.