Tacrolimus Monohydrate
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 1g | $550 | Check With Us |
| 2g | $850 | Check With Us |
| 5g | $1435 | Check With Us |
: Cat #: V3607 CAS #: 109581-93-3 (hydrate) Purity ≥ 98%
Description: Tacrolimus (formerly also known as FK-506), a macrocyclic lactone isolated from the culture broth of a strain of Streptomyces tsukubaensis, is an immunosuppressive agent used mainly after allogeneic organ transplant to reduce the activity of the patient's immune system and to lower the risk of organ rejection.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 822.05 |
|---|---|
| Molecular Formula | C44H71NO13 |
| CAS No. | 109581-93-3 (hydrate) |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: >100 mg/mLr |
| Water: <1 mg/mLr | |
| Ethanol: 83 mg/mL (103.2 mM) | |
| Synonyms | FR900506 hydrate; FR 900506 monohydrate; FR-900506; FK 506, FK-506, FK506, fujimycin, Prograf, Protopic, Advagraf; Astagraf XL; LCP-Tacro; |
| Protocol | In Vitro | Tacrolimus monohydrate (FK506 monohydrate; Fujimycin monohydrate; FR900506 monohydrate) inhibits calcium-dependent events, such as IL-2 gene transcription, NO synthase activation, cell degranulation, and apoptosis. Tacrolimus also potentiates the actions of glucocorticoids and progesterone by binding to FKBPs contained within the hormone receptor complex, preventing degradation. The agent may enhance expression of the TGFβ-1 gene in a fashion analogous to that demonstrated for CsA. T cell proliferation in response to ligation of the T cell receptor is inhibited by Tacrolimus. |
|---|---|---|
| In Vivo | The therapeutic effect of Tacrolimus is investigated on progression and perpetuation of colitis by administering Tacrolimus to Dextran sulfate sodium (DSS)-treated mice from Days 10 to 16 or to 23. At Days 17 and 24, colon length is significantly shortened, and colon weight is significantly higher in DSS-treated control animals than in normal animals. In addition, colon weight per unit length in the control group is more than twice that in the normal group. While both 7 and 14 d treatment with Tacrolimus significantly suppresses increases in colon weight per unit length in DSS-treated animals compared with the control group, this treatment does not actually restore the colon shortening. In addition, this inhibitory effect of Tacrolimus on increases in colon weight per unit length is more pronounced with 14-d than 7-d treatment, as shown by the inhibitory percentages (59% vs. 28%). |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.2165 mL | 6.0824 mL | 12.1647 mL | 24.3294 mL |
| 5mM | 0.2433 mL | 1.2165 mL | 2.4329 mL | 4.8659 mL |
| 10mM | 0.1216 mL | 0.6082 mL | 1.2165 mL | 2.4329 mL |
| 20mM | 0.0608 mL | 0.3041 mL | 0.6082 mL | 1.2165 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.