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NHI-2

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NHI-2
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Size Price Stock
100mg$980Check With Us
200mg$1470Check With Us
500mg$2480Check With Us
email: [email protected]        [email protected]

Cat #: V9332 CAS #: 1269802-97-2 Purity ≥ 99%

Description: NHI-2 is a novel, potent, selective and cell membrane permeable inhibitor of human lactate dehydrogenase isoform A (LDH-A; IC50 values 14.7 µM and 55.8 µM in a NADH competition assay for LDH-A and LDH-B, respectively), whcih is a key enzyme necessary to sustain glycolysis, the major pathway used by many cancer cells for cell growth and proliferation (the Warburg effect).

References: Granchi C, et al. Assessing the differential action on cancer cells of LDH-A inhibitors based on the N-hydroxyindole-2-carboxylate (NHI) and malonic (Mal) scaffolds. Org Biomol Chem. 2013;11(38):6588-6596.

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Molecular Weight (MW)335.28
Molecular FormulaC17H12F3NO3
CAS No.1269802-97-2
SMILES CodeO=C(C(N1O)=CC2=C1C=C(C3=CC=CC=C3)C=C2C(F)(F)F)OC 
SynonymsNHI-2; NHI 2; NHI2;
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.9826 mL14.9129 mL29.8258 mL59.6516 mL
5mM0.5965 mL2.9826 mL5.9652 mL11.9303 mL
10mM0.2983 mL1.4913 mL2.9826 mL5.9652 mL
20mM0.1491 mL0.7456 mL1.4913 mL2.9826 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.