5α-Cholestan-3β-ol (5α-Cholestanol; NSC 18188)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
50g	$400	Check With Us
100g	$650	Check With Us
200g	$975	Check With Us

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Cat #: V5160 CAS #: 80-97-7 Purity ≥ 98%

Description: 5α-Cholestan-3β-ol (5α-Cholestanol; Cholestanol; Dihydrocholesterol; NSC 18188), a reduced form of cholesterol at the 5,10-ene position, is derived from cholesterol by the action of intestinal microorganisms and can also be isolated from the testes of White Carneau pigeons.

References: M. T. Ravi Subbiah, et al. 5α-cholestan-3β-ol: High concentration in testis of white carneau pigeon. Lipids.1971 Jul, 6(7):517–519.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	388.67
Molecular Formula	C27H48O
CAS No.	80-97-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Synonyms	β-Cholestanol; (+)-Dihydrocholesterol; NSC18188; 5α-Cholestanol; Cholestanol; Dihydrocholesterol; NSC 18188; NSC-18188

Protocol	In Vitro	5α-Cholestan-3β-ol is derived from cholesterol by the action of intestinal microorganisms. It is known to induce the formation of gall stones in rabbits in the presence of sodium ions. 5α-Cholestan-3β-ol is used as a standard in lipid analysis using HPLC.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.5729 mL	12.8644 mL	25.7288 mL	51.4575 mL
5mM	0.5146 mL	2.5729 mL	5.1458 mL	10.2915 mL
10mM	0.2573 mL	1.2864 mL	2.5729 mL	5.1458 mL
20mM	0.1286 mL	0.6432 mL	1.2864 mL	2.5729 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.