SMCC crosslinker

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
1g	$150	Check With Us
5g	$450	Check With Us
10g	$675	Check With Us

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Cat #: V11269 CAS #: 64987-85-5 Purity ≥ 98%

Description: SMCC, formerly known as succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate, is a heterobifunctional protein crosslinker. SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.

References: [1]. Guo Y, et al. Potent antigen-specific immune response induced by infusion of spleen cells coupled with succinimidyl-4-(N-maleimidomethyl cyclohexane)-1-carboxylate (SMCC) conjugated antigens. Int Immunopharmacol. 2016 Feb;31:158-68.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	334.32
Molecular Formula	C16H18N2O6
CAS No.	64987-85-5
SMILES Code	O=C(C1CCC(CN2C(C=CC2=O)=O)CC1)ON3C(CCC3=O)=O
Synonyms	SMCC; SMCC crosslinker; heterobifunctional cross-linker;

Protocol	In Vitro	SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml.
Protocol	In Vivo	Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.9911 mL	14.9557 mL	29.9115 mL	59.8229 mL
5mM	0.5982 mL	2.9911 mL	5.9823 mL	11.9646 mL
10mM	0.2991 mL	1.4956 mL	2.9911 mL	5.9823 mL
20mM	0.1496 mL	0.7478 mL	1.4956 mL	2.9911 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.