PFK-158

This product is for research use only, not for human use. We do not sell to patients.

PFK-158
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250mg$750Check With Us
500mg$1280Check With Us
1g$1920Check With Us

Cat #: V2854 CAS #: 1462249-75-7 Purity ≥ 98%

Description: PFK-158 (formerly known as PFK158; ACT-PFK-158) is a first-in-class, potent and selective inhibitor of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFK-2/FBPase) isoform 3 (PFKFB3) with potential anticancer activity.

References: Mondal S, et al. Therapeutic targeting of PFKFB3 with a novel glycolytic inhibitor PFK158 promotes lipophagy and chemosensitivity in gynecologic cancers. Int J Cancer. 2019 Jan 1;144(1):178-189.

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Product Promise

Promise
Molecular Weight (MW)328.08
Molecular FormulaC18H11F3N2O
CAS No.1462249-75-7
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: ≥ 30 mg/mLr
Water: <1 mg/mLr
Ethanol: NA
SMILES CodeO=C(C1=CC=NC=C1)/C=C/C2=NC3=CC(C(F)(F)F)=CC=C3C=C2
SynonymsPFK-158; ACT-PFK-158; PFK 158; ACT-PFK158; PFK158; ACTPEK158.
ProtocolIn VitroPFK-158 (10 µM ; 24 hours; OV2008 and C13 cells) combined with Carboplatin (CBPt; 77-453 μM) results in significant increase in apoptosis in C13 (45%) and OV2008 cells (24.6%)
In VivoPFK-158 (15 mg/kg; intraperitoneal injection; once a week; for 4 weeks; female athymic nude mice) plus CBPt (51 mg/kg) treatment leads to significantly enhanced antitumor activity in a gynecologic cancer mouse model
Animal modelFemale athymic nude mice (nu/nu) (5-6 weeks old) injected with HeyA8MDR cells
Dosages15 mg/kg
AdministrationIntraperitoneal injection; once a week; for 4 weeks
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.0480 mL15.2402 mL30.4804 mL60.9607 mL
5mM0.6096 mL3.0480 mL6.0961 mL12.1921 mL
10mM0.3048 mL1.5240 mL3.0480 mL6.0961 mL
20mM0.1524 mL0.7620 mL1.5240 mL3.0480 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.